Abstract
Spot blotch, caused by Cochliobolus sativus, is a common foliar disease of barley that is controlled primarily through the development of resistant cultivars. An assay involving the inoculation of a detached first leaf (DFL) maintained in vitro was used to determine the reaction of five barley genotypes to the disease. The estimation of the infected leaf area enabled discrimination among barley genotypes differing in their susceptibility to the pathogen. Significant correlations were found (P=0.001) between in vitro DFL values in both seedling (r=0.89) and adult (r=0.95) plants. The established assay using in vitro DFL enables a fast assessment of the susceptibility of barley to spot blotch and should be useful for many types of studies on this disease.
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Arabi, M.I.E., Jawhar, M. Barley reaction to Cochliobolus sativus based on detached first leaf. Australasian Plant Pathology 36, 475–477 (2007). https://doi.org/10.1071/AP07053
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DOI: https://doi.org/10.1071/AP07053