Abstract
To identify molecular markers linked to the flax rust-resistance gene M4, RAPD analysis of NM4 (a near-isogenic line containing the M4 gene) and the recurrent parent Bison was performed using 540 decamer primers. The primer OPA18 amplified a specific fragment, OPA18432, in the NM4 line. The OPA18432 marker was found to be closely linked to the M4 gene, with a genetic distance of 2.1 cM, through the analysis of the F2 segregating population derived from a cross of BisonxNM4. Based on the sequence of OPA18432, the specific PCR primers were designed and aSCAR marker for the M4 gene was developed. The amplification of different resistant germplasm proved that the marker is specific for the M4 gene. This marker has been used successfully in marker-assisted selection in the flax breeding program.
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Bo, T.Y., Ma, J.J., Chen, J.X. et al. Identification of specific molecular markers linked to the rust resistance gene M4 in flax. Australasian Plant Pathology 37, 417–420 (2008). https://doi.org/10.1071/AP08032
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DOI: https://doi.org/10.1071/AP08032