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RAPD-PCR used to confirm that four pectic isozyme (zymogram) groups within the Australian Rhizoctonia solani AG 8 population are true intraspecific groups

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Abstract

RAPD-PCRs were used to study 79 isolates of Rhizoctonia solani AG 8 representing four pectic isozyme groups [zymogram (ZG)] (ZG1-1, 1-2, 1-4 and 1-5) from various locations in southern Australia. RAPD-PCR was performed on all isolates using six primers and the relatedness of the isolates determined by generating a neighbour joining tree. All isolates were also grouped according to vegetatively compatible populations (VCPs). Within each ZG there were four to nine VCPs. These studies show that the AG 8 population contains four distinct groups that matched the four ZGs and support the concept that ZGs are distinct intraspecific groups.

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Correspondence to P. A. O’Brien.

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MacNish, G.C., O’Brien, P.A. RAPD-PCR used to confirm that four pectic isozyme (zymogram) groups within the Australian Rhizoctonia solani AG 8 population are true intraspecific groups. Australasian Plant Pathology 34, 245–250 (2005). https://doi.org/10.1071/AP05004

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