American Journal of Orthodontics and Dentofacial Orthopedics
Original ArticleRevascularization after cryopreservation and autotransplantation of immature and mature apicoectomized teeth*,**
Section snippets
Material
The experimental material consisted of 16 single-rooted teeth, (incisors and first premolars) from 2 dogs of the same breed. The dogs were purebred and were 4 months 7 days old at the start of the experiment. Root development was complete in 12 teeth; 4 teeth had an open apex.15, 16, 17, 18
At the time of extraction, the pulp tissue was removed from the apical side of each tooth with the use of a nervebroche (Fig 1).An apicoectomy was
Results
The effect of 4 variables will be described: time, amount of revascularization, cryopreservation versus direct transplantation, and apicoectomy (Figs 3-5).
Discussion
Previous research has shown that the pulp tissue of replanted or autotransplanted teeth with partly formed roots becomes necrotic after the operation, and that revascularization occurs through the ingrowth of new, well-vascularized, cell-rich connective tissue.11, 12 Research by Skoglund and Tronstad,13, 14 showed that mature teeth with closed apexes also revascularize after autotransplantation when their root tips are cut, which enables the ingrowth of new blood vessels.
Until now, it has been
Conclusions
From this study, the following conclusions can be made:
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Teeth can revascularize after autotransplantation if the original pulp tissue is removed at the moment of extraction.
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There is no significant difference in the amount of revascularization between teeth stored in a tooth bank for 7 days and those immediately transplanted without freezing.
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There is no difference in the ingrowth of new pulpal tissue between mature apicoectomized teeth and immature teeth.
Acknowledgements
We wish to thank Mrs B. Jouret for typing the manuscript, Mr G. Dermout for the illustration, Dr B. De Smet, the director of the animal lab, and Dr I. De Cock for anesthetizing the animals.
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Cited by (37)
Microbial Factors and Antimicrobial Strategies in Dental Pulp Regeneration
2017, Journal of EndodonticsDental pulp tissue engineering and regenerative endodontic therapy
2017, Biomaterials for Oral and Dental Tissue EngineeringThe critical apical diameter to obtain regeneration of the pulp tissue after tooth transplantation, replantation, or regenerative endodontic treatment
2013, Journal of EndodonticsCitation Excerpt :Also, apicoectomized mature teeth with complete root development can be used. After apicoectomy, the teeth have an open apex and can be transplanted immediately at any age, keeping the possibility of revascularization (6–10, 15). By using mature apicoectomized teeth, the technique of tooth transplantation becomes less age-related.
Regional odontodysplasia: Orthodontic treatment and transplantation of premolars
2012, American Journal of Orthodontics and Dentofacial OrthopedicsPulpal regeneration and root development after subcutaneous transplantation of cryopreserved immature teeth in rats
2012, CryobiologyCitation Excerpt :After some time, tissue resembling bone or cementum occupied most of the pulp cavity which finally lead to obliteration. Laureys et al. [27] stated that the necrotic mass in the pulpal tissue after autotransplantation could be a stimulating factor in the repair process, because it was seen in their dog study that teeth with the pulpal tissues left in situ at the moment of transplantation showed more ingrowth of vital tissues compared to teeth transplanted with empty pulp chambers. The pulpal response to transplantation of the cryopreserved and control teeth could largely be divided into three different types: cementum-, bone- and dentin-like tissue formation.
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Reprint requests to: W. Laureys, Tandheelkunde, afd, Orthodontie, UZ - De Pintelaan 185 - P8, B - 9000 Gent, Belgium.
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Am J Orthod Dentofacial Orthop 2001;119:346-52