Role of mast cells in the onset of IgE-mediated late-phase cutaneous response in mice
Section snippets
Mice
Female specific-pathogen–free BALB/c Cr Slic mice were obtained from Japan SLC (Shizuoka, Japan) and used at 8 to 10 weeks of age. They were housed in plastic cages in an air-conditioned room at 24°C, fed a standard laboratory diet, and given water as desired. All experiments were performed according to a guideline for the care and use of experimental animals made by the Japanese Association for Laboratory Animal Science in 1987.
Antibodies and other reagents
Monoclonal anti-dinitrophenol IgE was prepared from EC-1 cells.6 A
Ear thickness
The changes of ear thickness in passively sensitized mice showed 2 peaks after DNFB challenge (Fig l, A ) (Fig 1, B ).figureThe first peak was at 1 hour, and the second was at 24 hours after the challenge, which represented the IPR and LPR, respectively.
Histologic findings
At 30 minutes to 1 hour after the DNFB challenge, slight dermal edema and vasodilatation were observed in the ears (Fig 2, A and B ).figureAt 4 and 8 hours, mild inflammatory cell infiltration (mainly neutrophils and a few macrophages) and focal
DISCUSSION
This study showed that AND and the expression of IL-6 mRNA and protein by mast cells within 4 hours after antigen challenge were important steps preceding the allergic cutaneous LPR in mice.
Allergic individuals, who are exposed to an appropriate antigen challenge, experience an IPR followed several hours later by the LPR. In recent years, investigators have focused on the LPR, because it is felt that this inflammatory response, which not only involves mediators but also mobilization of various
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