Clinical features of a polymorphism of the β ₃-adrenergic receptor gene in patients with type 2 diabetes mellitus - A study using a pin-point sequencing method

 

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Summary:

The human β ₃-adrenergic receptor (β ₃AR) is expressed specifically in adipose tissues, and its activation is activated in brown adipose tissues during thermogenesis and in white adipose tissues during lipolysis. We investigated the relationship between a polymorphism of the β ₃AR gene and the clinical features of type 2 diabetes mellitus. Studies were conducted in 30 type 2 diabetic patients (15 males and 15 females). Analysis of polymorphisms of the β ₃AR gene was performed by a pin-point sequencing method using the hair of the subjects. Preperitoneal (P-fat) and subcutaneous fat (S-fat) levels were determined by ultrasonography. We found a Trp⁶⁴Arg allele of the β ₃AR gene in the hair of 27% of all patients. The patients with this mutation showed a significantly younger onset-age of diabetes than those of the wild type. The body mass index, serum GPT levels, fasting immunoreactive insulin (IRI) and daily urinary C-peptide reaction (CPR) in the mutation group were markedly higher than in the wild type group. The P-fat, serum cholesterol and leptin concentrations tended to be higher in the mutation group. Patients in the mutation group had a significantly higher prevalence of hypertension (80%) compared with those in the wild type group (20%). Conclusions: The present results suggest that the clinical features of diabetic patients with a missense mutation in the β ₃AR gene are substantially distinct from those of the wild type patients. These specific features include obesity, hyperinsulinemia, hypertension, and an increase in preperitoneal fat.

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Toshihiko InukaiM.D., Ph.D. 

Department of Internal Medicine

Koshigaya Hospital

Dokkyo University School of Medicine

Minami-Koshigaya 2-1-50

Koshigaya, 343-8555

Japan

Phone: + 81-489-65-1111

Fax: + 81-489-65-1127