Identification of Volatile Constituents of Tambourissa leptophylla

 

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Abstract

The volatile fraction of Tambourissa leptophylla fruit skin was extracted by petrol ether, purified by adsorption chromatography (LPC) and analysed by gas chromatographic-spectroscopic methods. 27 non-oxygenated terpene hydrocarbons and 10 oxygenated derivatives were identified. The most abundant components were: limonene (24.0 %), cis-α-bergamotene (23.2 %), Δ-3-carene (8.2 %), α-curcumene (6.0 %), trans-α-bergamotene (5.1 %), α-copaene (4.1 %), α-pinene (4.0 %), p-cymene (4.0 %) and bicyclogermacrene (3.3 %). The crude volatile fraction was tested in vitro against Cladosporium cucumerinum in direct bioautography on TLC plates on the basis of the antifungal use of fruit skin. Activity of petrol ether extract against this micro-organism was demonstrated.

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Dr. Sandra Gallori

Dipartimento di Scienze Farmaceutiche

Università degli Studi di Firenze

Via Gino Capponi 9

50121 Firenze

Italy

Email: sandra.gallori@unifi.it

Fax: +39 055 240776