Laryngorhinootologie 2024; 103(S 02): S302-S303
DOI: 10.1055/s-0044-1785017
Abstracts │ DGHNOKHC
Otology/Neurootology/Audiology: Middle ear

Application of infrared light for detection of surgical relevant tissues during middle ear cholesteatoma surgery

Matthias Schürmann
1   Universitätsklinikum Bielefeld, Klinik für Hals-, Nasen-, Ohrenheilkunde, Bielefeld
,
Sylvia Steinecker
2   Universität Bielefeld, Physik, Bielefeld
,
Thomas Bischof
3   Nationales Zentrum für Tumorerkrankungen, Dresden
,
Caroline Berrou
3   Nationales Zentrum für Tumorerkrankungen, Dresden
4   Leibniz-Universität Hannover, Chemie, Hannover
,
Oliver Plettenburg
4   Leibniz-Universität Hannover, Chemie, Hannover
,
Thomas Huser
2   Universität Bielefeld, Physik, Bielefeld
,
Holger Sudhoff
1   Universitätsklinikum Bielefeld, Klinik für Hals-, Nasen-, Ohrenheilkunde, Bielefeld
,
Lars-Uwe Scholtz
1   Universitätsklinikum Bielefeld, Klinik für Hals-, Nasen-, Ohrenheilkunde, Bielefeld
,
Oliver Bruns
3   Nationales Zentrum für Tumorerkrankungen, Dresden
,
Ingo Todt
1   Universitätsklinikum Bielefeld, Klinik für Hals-, Nasen-, Ohrenheilkunde, Bielefeld
› Author Affiliations
› Further Information
Also available at
 

Recurrence after surgical extraction of middle ear cholesteatoma (MEC) is highly dependent on surgical techniques, specifically aggressiveness of the extraction. Clear visualization of residual MEC mass in the temporal bone would facilitate complete removal of the MEC mass. Regrowth from residual MEC mass is also associated with high level of inflammation/infection. Hence, removal of biofilm infected tissue is crucial in MEC surgery. The aim of this research endeavor is the construction of a prototype for a surgical microscope superimposing common microscopic image with newly developed contrasts based on infrared (IR) light. To acquire these contrasts, two approaches are pursued. The first one utilize IR light (700 nm – 1675 nm) to image tissues relevant in MEC surgery (temporal bone, MEC (N=10), muscle, tendon, fat). These experiments were performed on fixed human samples as well as fresh animal tissue. The second scope of the project is the development of fluorescent IR probes, able to stain bacterial biofilms. These probes are based on drugs and dyes already established in clinical routine and tested on various strains (N=9) isolated during MEC surgery. We were able to discriminate MEC from temporal bone, as well as tendon from muscle and fat using combinations of reflected IR light with different wavelength. It was also possible to demonstrate a distinct binding of our probes to the investigated strains. We think, that the label free contrast between tissues based on IR light as well as fluorescent probes based in clinical approved substance will make the translation of our methods feasible in short period of time. This might help ENT surgeon to optimize their performance and prevent recurrence of MEC.

Funding information Funding code: 13N15829 | Akronym: BetterView



Publication History

Article published online:
19 April 2024

© 2024. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany