Digital Evaluation of the Trueness and Fitting Accuracy of a Three-Unit Fixed Zirconium Bridge Fabricated from Different Types of Zirconia and Different Marginal Cement Space Thickness

Abstract Objective  The aim of this study was to measure the internal, marginal fitness, and trueness of a three-unit fixed zirconium bridge fabricated using two types of zirconia and different marginal cement space thicknesses. Methods and Materials  Thirty-two aluminum master models, constructed by computer numerical control (CNC) milling machines using aluminum rod, representing two prepared aluminum abutments (the upper first premolar and the first molar) were chosen due to differences in shape and dimensions, which may affect adaptation. It may also be considered one of the most common cases in clinical practice and a missing second premolars were used to fabricate a three-unit fixed partial zirconium bridge. A master model was scanned with an intraoral scanner. Fixed dental prostheses (FDPs) were fabricated with two designs (single-layer high-translucent [HT] Katana and ultra-translucent multilayer (UTML] zirconia Katana) and two marginal cement space thicknesses (0 and 25μm for HT and ML). These specimens are divided according to marginal cement space and type of zirconia into four groups, each containing eight samples. Group A: 25-μm marginal cement space with single-layer HT zirconia. Group B: 25-μm marginal gap with UTML zirconia. Group C: 0 μm marginal gap single-layer HT zirconia. Group D: 0 μm marginal gap with UTML zirconia. Fitness and trueness of the fabricated three-unit FDPs were evaluated by scanning the silicone replica of the cement space and analyzing the thickness of the silicone replica in the three-dimensional inspection software (Geomagic Control X, Morrisville, North Carolina, USA). Results  Highest mean value of internal gap was recorded at the molar abutment of group D (95.05, ± 3.42 μm), while the lowest was recorded at the premolar abutment of group A (57.12, ± 2.43μm). For marginal gap, the lowest marginal gap was also recorded at the premolar abutment of group A (36.14, ± 3.94μm), and the highest was at the molar abutment of group D (84.74, ± 2.98 μm). For trueness, group A showed the lowest root mean square (RMS) of mean with both abutments compared with group D, which recorded the highest RMS of mean for trueness. Conclusion  The marginal cement space thickness affects the FPD internal, marginal fitness, and trueness; single-layer HT (Katana) zirconia shows better fitness and trueness than UTML (Katana).


Introduction
The maintenance of prosthetic restorations is dependent upon achieving a high level of accuracy in terms of fit.2][3][4] The degree of marginal accuracy has a significant role in determining the success of a restoration in clinical performance.][7][8][9][10] The presence of an inaccurate inner surface of the prosthesis is among several factors that impact the marginal and internal fit.Therefore, it is imperative to conduct a trueness evaluation. 11,12The presence of internal fit disorders can have an impact on the long-term retention and resistance of the prosthesis.][15][16][17] All-ceramic systems have the potential to serve as viable substitutes for metal ceramic systems in the context of esthetic restorative materials for crowns and fixed partial dentures (FPDs). 18The utilization of zirconia in digital dentistry is experiencing a growing trend in popularity. 19,20irconia exhibits remarkable strength, thereby highlighting a limitation of conventional ceramic materials. 21To achieve aesthetic outcomes comparable to natural teeth, the application of veneering porcelain onto the zirconia framework is necessary.Nevertheless, a deficient configuration of the underlying structure and a significant disparity in the occlusal surface the occurrence of delamination and fracture of the veneering porcelain can be attributed to certain factors. 22he CAD/CAM system with ZrO2 material exhibited a satisfactory level of marginal fit. 23Recently, there have been advancements in the development of zirconia materials that exhibit high translucency and possess a multilayered structure capable of replicating the diverse range of colors observed in natural teeth, spanning from the cervical region to the incisal edge.These materials are commonly referred to as highly translucent (HT) zirconia and multilayer (ML) zirconia, respectively.Using these materials, it has become feasible to manufacture a visually pleasing monolithic zirconia restoration without the need for veneering porcelain.5][26][27][28] Nevertheless, there is a shortage of research examining the characteristics of ML zirconia with high translucency, and the accuracy of fit for fixed dental prostheses (FDPs) made from this material remains uncertain.There exist two distinct approaches for the production of zirconia FDPs: the first involves milling from a zirconia disk that has undergone complete sintering (fully sintered zirconia), while the second entails milling from a zirconia disk that has undergone partial sintering (pre-sintered zirconia).Presintered zirconia can be efficiently produced due to its comparatively modest flexural strength range of 31 to 50 MPa. 29The observed variation in the chemical composition of CAD/CAM materials appears to be correlated with the disparity in their postmilling firing requirements, which may in turn be indicative of the divergence in the marginal, internal, and overall fitness of the produced all ceramic crowns. 30In this study, we conducted an in vitro study to examine the impact of varying marginal cement gap thicknesses (0 μm, 25 μm) and two types of zirconia (HT and UTML Katana) on the trueness and FPDs fit.The factors contributing to the improved fit of FDPs are clarified.The null hypothesis stated that there would be no significant impact on the trueness and internal fit of threeunit fixed zirconium bridges when considering the marginal cement space thickness (0 μm, 25 μm) and two varieties of zirconia (HT, ML) used by the FDP.

Materials and Methods
This study employed an aluminum master die featuring two abutments, namely the maxillary first premolar and the first molar, as well as a single missing tooth, specifically the maxillary second premolar.The three-unit all-ceramic bridge was prepared with a 360 degree chamfer on each abutment tooth, as depicted in ►Fig. 1.An even application of dental scan spray (Optispray, Sirona Dental Systems GmbH, Bensheim, Germany) was administered to the upper dentoform arch, followed by the use of the InLab inEos X5 extra-oral scanner device (manufactured by Sirona Dental System, Bensheim, Germany) to capture the Standard Triangle Language (STL) file required for the design of a dental bridge.Two different types of zirconia, namely monolithic HT and UTML Katana , were manufactured.Subsequently, the determination of the thickness of the marginal cement space was conducted utilizing CAD software.The marginal gap of 0 μm was observed in two types of zirconia, namely HT and ML.Similarly, the marginal gap of 25 μm was also found in two types of zirconia, HT and ML; to get a cement space of 25 μm at the marginal area of the finishing line, the inlay/onlay parameter is utilized.The "marginal adhesive gap" is then selected and adjusted to a value of 25 μm.Next, the morphology of the crown should be adjusted, followed by the precise arrangement of the For trueness, group A showed the lowest root mean square (RMS) of mean with both abutments compared with group D, which recorded the highest RMS of mean for trueness.

Conclusion
The marginal cement space thickness affects the FPD internal, marginal fitness, and trueness; single-layer HT (Katana) zirconia shows better fitness and trueness than UTML (Katana).buttons.Finally, the "Edit restoration" option should be selected to let the program to compute all the necessary information before exporting it to the milling machine. 31

Trueness and Internal Fit Analysis
Using an optical scanner, specifically the Medit T710 desktop scanner manufactured in Seoul, South Korea, the inner surface of each abutment was scanned.Prior to each utilization, the scanner underwent calibration to guarantee accurate scanning.Every scan was converted into an STL file to be stored and used for the purpose of evaluating its accuracy 32 as shown in ►Fig.2.
The three-dimensional (3D) inspection software employed in this study was Geomagic Control X, developed by 3D Systems Inc., based in Rock Hill, South Carolina, United States.The Coping Designed Model (CDM) file and the Coping Scanned Model (CSM) file were imported into the software, and the two files were aligned through initial and best-fit alignment techniques.Subsequently, the 3D comparison feature was chosen to assess the disparities in dimensions between CDM and CSM.The established tolerance for this analysis was AE 10 µm, with a maximum and minimum range of AE 100 µm.Each data point within the inner region of each scan is comprised of three coordinates, namely X, Y, and Z.To determine the accuracy of the data, the Euclidean distance between each data point in the Control Data (CDM) and its corresponding point in the Comparison Data Set (CSM) files was computed. 15,33he utilization of root mean square (RMS) values is employed to characterize the standard deviation of the scanned data.A small RMS value indicates a high level of 3D agreement among the overlapping data.The color difference maps are presented for every 3D comparison in cases where the CSM file data are positioned above the CDM file data.In these maps, the red area represents the range of 10 to 100 µm.This implies that the manufacturing process failed to achieve the desired outcome.When the data in the CSM file is situated below the data in the CDM file, as denoted by the blue zone spanning from À10 to À100 µm, it implies that the manufacturing process has exceeded the intended position.The regions were precisely aligned with the green region, which measures less than 10 µm.If the RMS value is high, the color difference map will exhibit an increased presence of red and blue regions (►Fig. 3).This observation suggests that the 3D alignment of the overlay data is characterized by a suboptimal level of agreement.The Express Light Body, Fast Set, 3M ESPE material was utilized to fill the intaglio surface of the copings.Subsequently, the coping was placed on the model and subjected to finger pressure.Each coping was positioned on the model with an occlusal force of 5 kg (50 N).To simulate the biting forces generated by the mandible during the process of measurement, it is necessary to model those forces and for more standardization. 34,35The removal of the material after its complete polymerization from the die was subsequently undertaken. 36,37he inner surface of each coping, both with and without the impression material, was scanned using an optical scanner (Medit T710, Seoul, South Korea).The STL files obtained from the scans were saved and subsequently imported into the software Geomagic Control X, developed by 3D Systems Inc..These files were then overlaid with initial and best-fit alignment.The superimposed files were divided into hypothetical planes along the mesiodistal and buccolingual directions, and the fitness was assessed along these planes.In each sample, a total of 600 points were systematically chosen along the theoretical planes, with equal spacing between the points within each sample (►Fig.4).The data analysis was conducted using IBM SPSS Statistics, version 26.0 (IBM Corp., Armonk, New York, United States).The statistical tests employed for assessing significant differences between the groups were one-way analysis of variance (ANOVA) and Tukey's honestly significant difference test.

Results
The mean RMS value for trueness was the highest for the molar abutment of group D (63.25, AE 3.67 μm), while the lowest RMS value was for trueness for the premolar abutment of group A (29.63, AE 5.13 μm) as shown in ►Table 1.
For the internal fit, the premolar abutment of group A showed the lowest mean of internal gap (57.12,AE 2.43μm), while the highest mean of internal gap was recorded at the molar abutment of group D (95.05, AE 3.42 μm).And for the marginal gap, also the molar abutment of group D had the highest mean value (84.74,AE 2.98 μm) and the lowest mean  for marginal was recorded for the premolar of the abutment of group A (36.14, AE 3.94μm) and the highest mean marginal fitness was recorded for the molar abutment of group D (84.74, AE 2.98μm) showed in ►Table 2.
The results of the one-way ANOVA test revealed a statistically high significant difference at a significance level of p ¼ 0.000 for both the mean RMS value of trueness and the mean internal gaps.The Tukey's test was conducted to perform multiple comparisons between various pairs of groups to investigate the underlying source of the observed differences.The Tukey's test showed there is statistically highly significant difference between all groups in the marginal and internal fitness.Except molar abutment of group C and molar abutment of group D there was nonsignificant difference.As for mean RMS values trueness, there is statistically nonsignificant difference between group A and except between premolar and molar abutments of group A is statistically significant difference and between premolar of group A and molar abutment of group B there is statistically high significant difference.A high significant difference between group C and D except between premolar and molar abutments of group C is statistically significant difference.Between molar abutment of group C and premolar abutment of group D is nonsignificant difference.

Discussion
The null hypothesis was rejected due to the presence of a statistically significant difference in trueness (p ¼ 0.000) and fitness (p ¼ 0.000) between two types of zirconia HT and ML fabricated with different types of cement space thickness (0 and 25 μm).

Internal Fit
In this study, the results showed that the FDPs' design and different cement space thickness (0-25μm) had an effect on the marginal and internal fit when compared with the results obtained when we used a 0 μm cement space at the marginal area of preparation.The results of this study showed that using a 25μm cement space around the marginal area of preparation results in a thinner cement film thickness than the set cement space at all areas of measurement according to the mean marginal gap of premolar and molar abutments, as groups A and B had the lowest mean marginal gap than C and D, as shown in ►Table 2. This may be explained by the fact that by using a 25μm cement space at the margin, the frictional resistance that results from the seating of crown restorations may be reduced, and this is agreed with. 38,39We thought about the possibility that the ML structure of zirconia could cause a change in size.By including FDPs made from single-layer HT zirconia in the experimental group, we could see how the ML structure affected the marginal and internal fit.The single-layer HT zirconia with a marginal gap of 25μm in group A had the lowest mean marginal and internal gap for both abutments premolar and molar as when compared with UTML zirconia with the same marginal gap of 25 μm, which was also the same for Groups C and D, which were fabricated with the same marginal cement space thickness 0μm and from different types of zirconia (HT for Group C and UTML for Group D), which revealed that Group C had a lower marginal and internal gap than Group D. Thus, these groups fabricated from single layer HT zirconia had a better fit than groups fabricated from ML zirconia, and this happens because the amounts of metal oxide particles in each layer are different, and the amount and timing of shrinkage in each layer during secondary firing differed.This could have resulted in layer distortion.Because the company did not provide the authors with any information regarding the coloring particles, the influence of various metal oxides and their amount on the fit cannot be revealed. 40urthermore, it is believed that the process utilized to create the ML zirconia affects the degree of distortion.Therefore, the decreased trueness can be ascribed to various factors, including the dimensions and configuration of the milling burs, the quality of the CAD data collected by the CAM system, and the processing capabilities.The formation of rounded edges is a consequence of the limited resolution of the CAD-CAM imaging system, as well as the challenges associated with scanning acute angles during the image acquisition process and subsequent reconstruction by the software program.During the process of reading, the phenomenon of light reflection is observed to occur with greater intensity compared with flat areas and this agreed with other Majeed ZA and Jasim HH in 2023. 33

Conclusion
Given this study's limitations, the findings showed that: 1. HT Katana zirconia had the lowest mean RMS of trueness and the lowest internal and marginal gap, which means it had the least amount of deviation from the design and a better fit.2. ML Katana Zirconia had a greater amount of deviation from the design due to its highest mean RMS for trueness and high mean internal and marginal gaps.3. Marginal cement space thickness affects the internal fit; a 25 μm marginal cement space thickness showed a better fit than a 0 μm.

Fig. 1 Fig. 2
Fig.1An aluminum master die featuring two abutment teeth, specifically the maxillary first premolar and first molar, as well as a vacant space in the second premolar region.

Fig. 3
Fig. 3 Color difference map with three-dimensional compare option.Positive deviations are visually represented by a color spectrum ranging from yellow to red, whereas negative deviations are visually represented by a color spectrum ranging from cyan to blue.

Fig. 4
Fig.4The assessment of marginal and internal fit within a theoretical plane.

Table 1
Mean RMS value (μm) for trueness of the four groups Abbreviations: RMS, root mean square; SD, standard deviation.

Table 2
Mean internal and marginal gaps (μm) of the four groups Abbreviation: SD, standard deviation.

41 Trueness
According toCho etal 2019, the acceptable RMS value of the trueness is below 50μm; so based on the results of this study, the mean RMS values for trueness were 29.63, AE 5.13μm for premolar abutment and 35.61,AE 3.76μm for molar abutment of group A and for group B, RMS values were 32.94, AE 2.72μm for premolar abutment and 36.73,AE 2.69 μm for abutment; while in group C only premolar abutment was below the acceptable range, which was 47.90, AE 4.04 μm, though molar abutment showed greater RMS value of 54.16, AE 4.46 μm, and in group D, RMS values for premolar and molar were 55.25, AE 2.99 μm and 63.25, AE 3.67 μm, respectively, were above the accepted range; so these groups had a greater deviation from the design.