Planta Med 2017; 83(14/15): 1117-1129
DOI: 10.1055/s-0043-113448
Reviews
Georg Thieme Verlag KG Stuttgart · New York

DNA Barcoding for Industrial Quality Assurance

Tiziana Sgamma
1   Biomolecular Technology Group, School of Allied Health Sciences, De Montfort University, Leicester, UK
,
Claire Lockie-Williams
2   BP-NIBSC Herbal Laboratory, National Institute for Biological Standards and Controls, Potters Bar, UK
,
Marco Kreuzer
2   BP-NIBSC Herbal Laboratory, National Institute for Biological Standards and Controls, Potters Bar, UK
4   Section of Ecology and Evolutionary Biology, School of Biological Sciences, University of Reading, Reading, UK
,
Sarah Williams
1   Biomolecular Technology Group, School of Allied Health Sciences, De Montfort University, Leicester, UK
,
Ulrike Scheyhing
3   Dr. Willmar Schwabe Pharmaceuticals, Karlsruhe, Germany
,
Egon Koch
3   Dr. Willmar Schwabe Pharmaceuticals, Karlsruhe, Germany
,
Adrian Slater
1   Biomolecular Technology Group, School of Allied Health Sciences, De Montfort University, Leicester, UK
,
Caroline Howard
1   Biomolecular Technology Group, School of Allied Health Sciences, De Montfort University, Leicester, UK
2   BP-NIBSC Herbal Laboratory, National Institute for Biological Standards and Controls, Potters Bar, UK
› Author Affiliations
Further Information

Publication History

received 10 March 2017
revised 16 May 2017

accepted 27 May 2017

Publication Date:
29 June 2017 (online)

Abstract

DNA barcoding methods originally developed for the identification of plant specimens have been applied to the authentication of herbal drug materials for industrial quality assurance. These methods are intended to be complementary to current morphological and chemical methods of identification. The adoption of these methods by industry will be accelerated by the introduction of DNA-based identification techniques into regulatory standards and monographs. The introduction of DNA methods into the British Pharmacopoeia is described, along with a reference standard for use as a positive control for DNA extraction and polymerase chain reaction (PCR). A general troubleshooting chart is provided to guide the user through the problems that may be encountered during this process. Nevertheless, the nature of the plant materials and the demands of industrial quality control procedures mean that conventional DNA barcoding is not the method of choice for industrial quality control. The design of DNA barcode-targeted quantitative PCR and high resolution melt curve tests is one strategy for developing rapid, robust, and reliable protocols for high-throughput screening of raw materials. The development of authentication tests for wild-harvested Rhodiola rosea L. is used as a case study to exemplify these relatively simple tests. By way of contrast, the application of next-generation sequencing to create a complete profile of all the biological entities in a mixed herbal drug is described and its potential for industrial quality assurance discussed.

Supporting Information

 
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