CC BY 4.0 · Aorta (Stamford) 2022; 10(S 01): A1-A56
DOI: 10.1055/s-0042-1750975
Presentation Abstracts

Study of Heart Stem Cells in Cardiac Surgery Patients of Different Ages

Veronika Velichko
1   First Pavlov State Medical University, Saint-Petersburg, Russia
,
Aleksander Nemkov
1   First Pavlov State Medical University, Saint-Petersburg, Russia
,
Galina Belostotskaya
2   Sechenov Institute of Evolutional Physiology and Biochemistry RAS, Saint-Petersburg, Russia
,
Alexandr Kriventsov
1   First Pavlov State Medical University, Saint-Petersburg, Russia
,
Vladimir Komok
1   First Pavlov State Medical University, Saint-Petersburg, Russia
,
Nikolay Bunenkov
1   First Pavlov State Medical University, Saint-Petersburg, Russia
,
Vadim Karev
3   Research Institute of Children's Infections, Saint-Petersburg, Russia
,
Gulfiya Nutfullina
1   First Pavlov State Medical University, Saint-Petersburg, Russia
,
Gennady Khubulava
1   First Pavlov State Medical University, Saint-Petersburg, Russia
› Author Affiliations
› Further Information
 

Background: Little is known about heart stem cells. It is believed that there is no regeneration in patients older than 75 years Goal. The study of heart stem cells in patients of different age.

Methods: 24 patients underwent cardiac surgery. The age of patients is from 47 to 80 years. A right atrium auricle myocardial biopsy was performed for everyone from the center of the purse string suture immediately before the introduction of a venous cannula. The following studies were performed: histology, immunohistochemistry, enzyme treatment followed by cultivation to obtain an organ-specific progenitor cell culture, confocal microscopy.

Results: Stem cells were not convincingly detected in myocardial samples during histological and immunohistochemical examination. Cardiac stem cells with the c-kit marker were found in myocardial samples from an organ-specific progenitor cell culture during confocal microscopy in all cases. Cell-in-cell structures were also found in all samples, including 78-79-80-year-old patients.

Conclusion: The heart stem cells and cell-in-cell structures were found in cardiac surgery patients of different ages, including 80-year-olds. The number of structures was greater in younger patients.

References

1. Belostotskaya G, Nevorotin A, Galagudza M. Identification of cardiac stem cells within mature cardiac myocytes. Cell Cycle 2015;14(19):3155–3162 10.1080/15384101.2015.1078037 PubMed

2. Belostotskaya GB, Golovanova TA. Characterization of contracting cardiomyocyte colonies in the primary culture of neonatal rat myocardial cells: a model of in vitro cardiomyogenesis. Cell Cycle 2014;13(6):910–918 10.4161/cc.27768 PubMed

3. Belostotskaya G, Hendrikx M, Galagudza M, Suchkov S. How to Stimulate Myocardial Regeneration in Adult Mammalian Heart: Existing Views and New Approaches. BioMed Res Int 2020;2020:7874109 10.1155/2020/7874109 PubMed

4. Belostotskaya GB, Nerubatskaya IV, Galagudza MM. Two mechanisms of cardiac stem cell-mediated cardiomyogenesis in the adult mammalian heart include formation of colonies and cell-in-cell structures. Oncotarget 2018;9(75):34159–34175 PubMed



Publication History

Article published online:
10 June 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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