Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596819
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Exploitation of agro-industrial by-products for recovery of bioactive compounds with applications in pest management

P Vlachou
1   Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
,
M Holeba
2   Benaki Phytopathological Institute, St. Delta 8, 14561, Kifissia, Greece
,
A Termentzi
1   Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
2   Benaki Phytopathological Institute, St. Delta 8, 14561, Kifissia, Greece
,
E Markellou
2   Benaki Phytopathological Institute, St. Delta 8, 14561, Kifissia, Greece
,
N Ntalli
2   Benaki Phytopathological Institute, St. Delta 8, 14561, Kifissia, Greece
,
LA Skaltsounis
1   Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
,
N Fokialakis
1   Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Concerns over the potential environmental impact of synthetic pesticides have led to the development of natural product-based pesticides. Food and forestry industries annually produce large amounts of by-products worldwide, which are characterized by their high contents of bioactive compounds that could serve as alternatives in crop protection. In the present study, 20 extracts and 4 hydrosols, by-products from wood (Pinus nigra, Pinus heldreichii, Eucalyptus globulus and Juniperus phoenicea) and food-juice industries (Punica granatum and Prunus persica) were tested for their ability to control phytopathogenic organisms (antibacterial, antifungal and nematicidal action). The samples were tested against 17 bacteria of the genera Acidovorax, Agrobacterium, Erwinia, Pseudomonas and Xanthomonas (disk diffusion assay), 2 fungal strains of Phytophthora nicotianae (agar dilution assay) and the nematode Meloidogyne javanica (larvae paralysis capacity assay). P. granatum extracts showed good control against Pseudomonas corrugata, Erwinia carotovora pv. atroseptica and all tested species of Xanthomonas and Acidovorax, while E. globulus extracts inhibited the growth of X. campestris pv. campestris at a concentration of 1 mg/disk. These extracts also revealed strong activity against both strains of P. nicotianae in a concentration of 10 mg/mL. Finally, all P. persica extracts were effective against M. javanica at a concentration of 1500 µg/mL (paralysis rate 61%). The active extracts were investigated by LC-HRMS/MS for identification of their major constituents. E. globulus extracts were rich in proanthocyanidins and ellagitannins. flavonoids, triterpeniodsand phenolic acids were the major constituents of P. persica extracts, while P. granatum extracts were characterized by punicalagins, anthocyanidins, gallic acid and flavonoids. These by-products could serve as potential, cost effective source of valuable compounds, which could be used for the development of biopesticides.

Keywords: agricultural by-products, antibacterial, antifungal, nematicidal activity, LC-HRMS/MS, biopesticides.