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DOI: 10.1055/s-0031-1282232
Ultra-performance liquid chromatographic (UPLC) determination of the rutin and chlorogenic acid in the Ribes anatolica and its antioxidant activity
A new ultra performance liquid chromatographic (UPLC) method was developed for the determination of rutin and chlorogenic acid in Ribes anatolica Behçet (Grossulariaceae) which is an endemic species in Turkey [1]. Good chromatographic separation and determination were performed on an Acquiting UPLCTM BEH phenyl column (100mm x 1.0mm, i.d., 1.7µm) system by using a mobile phase containing acetonitrile and formic acid buffer solution (pH=3.77) with 3% triethylamine (15:85) (v/v) (See Figure 1).
Chromatographic separation is by an isocratic elution with the flow rate of 0.3 mL/min. Calibration graphs for both compounds in the linear concentration range of 5–40µg/mL were obtained by using the relationship between the concentration and the peak area based on the detection at 340nm. The validity of the UPLC method was done by analyzing the plant samples. The developed UPLC method was applied to the quantitative analysis of R. anatolica consisting of rutin and chlorogenic acid and a good agreement was reported. The estimation of the antioxidant activities of water and methanol extracts of the leaf is based on thiobarbituric acid (TBA) assay in order to detect their liposome lipid peroxidation. In this investigation, the significant activities were obtained from the water (IC50=24.85±6,33) and MeOH (IC50=27.03±6,64) extracts of R. anatolica in the TBA test. Propyl gallate (IC50=0.04±0,18) was used as a positive control.
References: 1. Behçet L (2001) Turk J Botany 25: 103–105.