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Planta Med 2024; 90(01): 13-24
DOI: 10.1055/a-2181-2569
Biological and Pharmacological Activity
Original Papers

Tapirira guianensis is Selectively Cytotoxic, Induces Apoptosis to the Glioblastoma and Decreases Tumor Growth and Angiogenesis in vivo

Ana Gabriela Silva Oliveira
1   Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Marina Andrade Rocha
1   Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Lucas Santos de Azevedo
1   Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Aline Thaynara de Moura Coelho
1   Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Rafael César Russo Chagas
1   Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Hélio Batista Santos
2   Tissue Processing Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Ralph Gruppi Thomé
2   Tissue Processing Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
,
Peter Samuel
3   Zurich University of Applied Sciences, Department of Life Sciences and Facility Management, Wädenswil, Switzerland
,
Evelyn Wolfram
3   Zurich University of Applied Sciences, Department of Life Sciences and Facility Management, Wädenswil, Switzerland
,
Bonglee Kim
4   Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea
,
Rui Manuel Reis
5   Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil
6   Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Portugal
,
Rosy Iara Maciel Azambuja Ribeiro 
1   Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil
› Author AffiliationsThe authors are grateful for the research support provided by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG – PPM-00229-16 and APQ-00068-18) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES – Finance Code 001). This research was also supported by FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/2013, FPXII-BIOPLAT). Furthermore, we thank all other parties who made this study possible.
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Abstract

Glioblastoma is the most frequent primary malignant brain tumor without effective treatment, which makes this work extremely relevant. The study of the bioactive compounds from medicinal plants plays an important role in the discovery of new drugs.

This research investigated the constituents of Tapirira guianensis and its antitumor potential (in vitro and in vivo) in glioblastoma. The T. guianensis extracts were characterized by mass spectrometry. The ethyl acetate partition (01ID) and its fractions 01ID-F2 and 01ID-F4 from T. guianensis showed potential antitumor treatment evidenced by selective cytotoxicity for GAMG with IC50 14.1 µg/mL, 83.07 µg/mL, 59.27 µg/mL and U251 with IC50 25.92 µg/mL, 37.3 µg/mL and 18.84 µg/mL. Fractions 01ID-F2 and 01ID-F4 were 10 times more selective when compared to TMZ and 01ID for the two evaluated cell lines. T. guianensis also reduced matrix metalloproteinases 2 – 01ID-F2 (21.84%), 01ID-F4 (29.6%) and 9 – 01ID-F4 (73.42%), ID-F4 (53.84%) activities, and induced apoptosis mainly through the extrinsic pathway. Furthermore, all treatments significantly reduced tumor size (01ID p < 0,01, 01ID-F2 p < 0,01 and 01ID-F4 p < 0,0001) and caused blood vessels to shrink in vivo. The present findings highlight that T. guianensis exhibits considerable antitumor potential in preclinical studies of glioblastoma. This ability may be related to the phenolic compounds and sesquiterpene derivatives identified in the extracts. This study deserves further in vivo research, followed by clinical investigation.

Key words

Glioblastoma - cerrado - Tapirira guianensis - Anacardiaceae - apoptosis - angiogenesis

Supporting Information

    The effects used in the Western blotting assay and their concentrations are described in the supplemental material.

  • Supporting Information


Publication History

Received: 05 April 2023

Accepted after revision: 19 September 2023

Article published online:
13 October 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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