Simultaneous Determination of Diastereomers (+)-Licarin A and Isolicarin A from Myristica fragrans in Rat Plasma by HPLC and its Application to their Pharmacokinetics

 

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Abstract

A rapid and simple high-performance liquid chromatographic (HPLC) method was developed and validated to simultaneously analyze the diastereomers of (+)-licarin A and isolicarin A in rat plasma after intravenous administration. The analytes were extracted from the plasma by solid-phase extraction (SPE). Diastereomeric separation and determination were successfully achieved using a Diamonsil™ ODS C₁₈ reversed-phase column (250 mm × 4.6 mm i. d., 5 μm) with an RP18 guard column (8 mm × 4.6 mm i. d., 5 μm) and a mobile phase of MeOH-H₂O (4 : 1, v/v). UV detection was at 270 nm. The linear ranges of the standard curves were 0.25 – 150.00 μg/mL for (+)-licarin A and 0.10 – 75.00 μg/mL for isolicarin A. The lower limits of detection and quantification were 0.05 and 0.25 μg/mL for (+)-licarin A, and 0.05 and 0.10 μg/mL for isolicarin A, respectively. This assay method was successfully applied to study the pharmacokinetics of diastereomers (+)-licarin A and isolicarin A in rat plasma.

Abbreviations

HPLC:high-performance liquid chromatography

LLOD:lower limits of detection

LLOQ:lower limits of quantification

QC:quality control

R.S.D.:relative standards deviation

SD:Sprague-Dawley

SPE:solid-phase extraction

UV:ultraviolet

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Prof. Xiu-Wei Yang

State Key Laboratory of Natural and Biomimetic Drugs

Department of Natural Medicines

School of Pharmaceutical Sciences

Peking University

Beijing 100083

People’s Republic of China

Phone: +86/10/8280/5106

Fax: +86/10/8280/2724

Email: xwyang@bjmu.edu.cn

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