Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596437
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic, anti-radical and reducing properties of ethanol bark extracts of Geissospermum reticulatum

JJ Sajkowska-Kozielewicz
1   Department of Physical Chemistry, Medical University of Warsaw, Banacha 1, 02 – 097 Warsaw, Poland
,
P Kozielewicz
2   School of Clinical and Experimental Medicine, University of Birmingham, Edgbaston, B15 2TT Birmingham, UK
,
NM Barnes
2   School of Clinical and Experimental Medicine, University of Birmingham, Edgbaston, B15 2TT Birmingham, UK
,
I Wawer
1   Department of Physical Chemistry, Medical University of Warsaw, Banacha 1, 02 – 097 Warsaw, Poland
,
K Paradowska
1   Department of Physical Chemistry, Medical University of Warsaw, Banacha 1, 02 – 097 Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

This study has been undertaken as a part of a research project on the phytochemistry and biological activities of Amazonian plant Geissospermum reticulatum A. H. Gentry (family Apocynaceae). This plant is commonly used by traditional healers in South America to treat general illness, inflammation, wounds and malaria. To this date, only a limited number of studies have been published on G. reticulatum. [1, 2] This work reports for the first time cytotoxic, anti-radical and reducing activities of seven samples of G. reticulatum bark collected in Peru. The ethanolic extracts of G. reticulatum were evaluated in DOHH2 and HEK293 cells for their cytotoxicity using 7-amino-actinomycin D (7-AAD). The ferric reducing ability of plasma (FRAP) assay and fluorimetric oxygen radical absorbance capacity (ORAC) assay were employed to determine anti-radical activities of the extracts. All extracts showed cytotoxic properties for both cell lines with IC50 values of ca. 0.2 µg/ml. The ethanolic extracts 7, 3 and 2 showed the highest antioxidant capacity values as measured by the ORAC assay: 86.95, 83.47 and 80.57µmol trolox equivalents/l, respectively. The values of the reducing power of the studied extracts varied from 3.86 to 7.28 mmol/l. We observed the relationship between the results of cytotoxicity and the ORAC assay: DOHH2 vs. ORAC (R 2= 0.84) and HEK293 vs. ORAC (R 2= 0.76). Our results indicate that G. reticulatum is rich in antioxidants and exhibits cytotoxic properties. Interestingly, the cytotoxicity of the extracts was proportional to their antioxidant capacity, and these properties may arise from the presence of the same constituents. This study provides valuable information supporting potential therapeutic use of G. reticulatum bark. Further work is ongoing to isolate the active ingredients of the extract and elucidate their mechanism of action.

Keywords: Geissospermum reticulatum, Apocynaceae, cytotoxicity, anti-radical activity.

References:

[1] Gentry AH. New species and combinations in Apocynaceae from Peru and adjacent Amazonia. Ann Missouri Bot Gard 1984; 1075 – 1081

[2] Gordillo-Román B, Reina M, Ruiz-Mesia L, Ruiz-Mesia W, Joseph-Nathan P. Absolute configuration of indoline alkaloids from Geissospermum reticulatum. Tetrahedron Lett 2013; 54: 1693 – 1696