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Planta Med 2003; 69(5): 479-481
DOI: 10.1055/s-2003-39700
Letter
© Georg Thieme Verlag Stuttgart · New York

Embryoidogenesis and Plant Regeneration from Leaf Tissue of Gloriosa superba

G. Sivakumar1 , K. V. Krishnamurthy2 , T. D. Rajendran3
  • 1CIRASAIA, Dipartimento di Chimica, Università della Calabria, Arcavacata di Rende, Italy
  • 2Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India
  • 3Herbs and Drugs India, Sivakasi, Tamilnadu, India
Further Information

Publication History

Received: September 13, 2002

Accepted: December 15, 2002

Publication Date:
12 June 2003 (online)

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Abstract

The induction, maturation and germination of embryoids from leaf tissue of Gloriosa superba L. were developed by exploiting solid and liquid culture. Nodular calli were obtained from SH medium supplemented with 2,4-D and 2iP. In solid culture, the nodular calli when transferred to 2,4-D along with glycerol gave the best response (68.4 %) in embryoid induction after 20 days. After two subcultures at 7-day intervals in a medium with thiamine instead of glycerol, the embryoids matured. When mature embryoids were transferred to BAP and IBA medium, they gave rise to plantlets with single shoots and roots. In liquid culture, the medium supplemented with NAA and L-glutamine with continuous agitation, the embryoidogenic calli produced embryoids (85 %) after 21 days. The mature embryoids began to turn green and produced shoots and elongated ”radicles” after 35 days.

Abbreviations

SH:Schenk and Hildebrand (1972)

Kin:Kinetin

BAP:6-Benzylamino purine

2iP:N 6-(2-isopentyl)adenine

IBA:Indole-3-butyric acid

NAA:α-Naphthaleneacetic acid

2,4-D:2,4-Dichlorophenoxyacetic acid

References

  • 1 Sivakumar G, Krishnamurthy K V. Gloriosa superba L. - a very useful medicinal plant, Recent Progress in Medicinal Plants, Vol. 7 - Ethnomedicine and Pharmacognosy Part II. In: Singh VK, Govil JN, Hashmi S, Singh G, editors Series. Sci Tech Pub Texas, U.S.A; 2002: pp. 465-82
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  • 2 Sivakumar G, Krishnamurthy K V. Micropropagation of Gloriosa superba L. - an overexploited medicinal plant species from India. Role of Plant Tissue Culture in Biodiversity Conservation and Economic Development. In: Nandi SK, Palni LMS, Kumar A, editors Gyanodaya prakashan pub Nainital, India; 2002: pp. 345-50
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  • 3 Schenk R U, Hildebrandt A C. Medium and techniques for induction and growth monocotyledonous and dicotyledonous plant cell cultures.  Can J Bot. 1972;  50 199-204
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Dr. G. Sivakumar

CIRASAIA

Dipartimento di Chimica

Università della Calabria

Via Pietro Bucci Cubo 15C

87036 Arcavacata di Rende

Italy

Email: sivakumar@libero.it

Fax: +39-0984-492116

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