Activity-Guided Fractionation of the Seeds of Ziziphus jujuba Using a Cyclooxygenase-2 Inhibitory Assay

 

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Abstract

Bioactivity-guided fractionation of petroleum ether- and EtOAc-soluble extracts of the seeds of Ziziphus jujuba using a cyclooxygenase-2 assay as a monitor indicated that the triglyceride, 1,3-di-O-[9(Z)-octadecenoyl]-2-O-[9(Z),12(Z)-octadecadienoyl]glycerol (3), and a fatty acid mixture of linoleic, oleic and stearic acids, were the major active components. A new pentacyclic lupane-type triterpene derivative, 3-O-[9(Z)-octadecenoyl]betulinic acid (1), and betulinic acid (2) were also isolated and identified. All isolates as well as pure linoleic, oleic and stearic acids were evaluated for their inhibitory effects against both cyclooxygenases-1 (COX-1) and -2 (COX-2).

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Prof. A. Douglas Kinghorn, Ph. D., D.Sc.

Program for Collaborative Research in the Pharmaceutical Sciences and

Department of Medicinal Chemistry and Pharmacognosy

College of Pharmacy

University of Illinois at Chicago

833 South Wood Street (M/C 781)

Chicago, IL 60612

USA

Phone: +1-312-996-0914

Fax: +1-312-996-7107

Email: kinghorn@uic.edu