Thromb Haemost 2001; 85(05): 896-902
DOI: 10.1055/s-0037-1615765
Review Article
Schattauer GmbH

7E3 F(ab’)2, an Effective Antagonist of Rat αIIbβ3 and αvβ3, Blocks In Vivo Thrombus Formation and In Vitro Angiogenesis

Patricia M. Sassoli
1   Biology Research Centocor, Malvern, USA
,
Eva L. Emmell
1   Biology Research Centocor, Malvern, USA
,
Susan H. Tam
1   Biology Research Centocor, Malvern, USA
,
Mohit Trikha
1   Biology Research Centocor, Malvern, USA
,
Zhao Zhou
1   Biology Research Centocor, Malvern, USA
,
Robert E. Jordan
1   Biology Research Centocor, Malvern, USA
,
Marian T. Nakada
1   Biology Research Centocor, Malvern, USA
› Author Affiliations
Further Information

Publication History

Received 17 August 2000

Accepted after resubmission 28 December 2000

Publication Date:
11 December 2017 (online)

Summary

Abciximab (c7E3 Fab, ReoPro®) blocks GPIIb/IIIa and αvβ3 and inhibits thrombotic and proliferative events only in humans and non-human primates. The bivalent F(ab’)2 fragment is an effective anti-thrombotic agent in canine models. In the present study, 7E3 F(ab’)2 was also found to bind to rat GPIIb/IIIa (KD = 27 ± 4 g/mL) and αvβ3 (KD = 9 ± 8 μg/mL), to block in vitro rat platelet aggregation (IC50 = 16 ± 6 μg/mL), and to inhibit αvβ3-mediated microvessel sprout formation in a rat aortic ring assay. Following administration of 7E3 F(ab’)2 (4 mg/kg) to rats, platelet aggregation was completely blocked for up to 6 h and thrombus formation in response to a rat abdominal aorta double crush injury was prevented. Effective chronic dosing was achieved with 6 mg/kg daily I.P. injections. In vitro mixing experiments indicated that 7E3 F(ab’)2 redistributed to unlabeled platelets in 2 h. Ex vivo, 7E3 F(ab’)2 was detected on platelets for up to 4 days after a single 4-mg/kg injection. These data suggest that 7E3 F(ab’)2 may be a useful agent to study the effects of GPIIb/IIIa and αvβ3 blockade in rat models of thrombosis and vascular disease.

 
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