Age-related alterations in caffeine-sensitive calcium stores and mitochondrial buffering in rat basal forebrain

doi:10.1054/ceca.1999.0048

Cell Calcium

Volume 25, Issue 6, June 1999, Pages 439-452

https://doi.org/10.1054/ceca.1999.0048 Get rights and content

Abstract

The properties of caffeine- and thapsigargin-sensitive endoplasmic reticulum calcium stores were compared in acutely dissociated basal forebrain neurons from young and aged F344 rats by ratiometric microfluorimetry. The ability of these stores to sequester and release calcium resembles that observed in other central neurons, with an important role of mitochondrial calcium buffering in regulating the response to caffeine. An age-related reduction in the filling state of the stores in resting cells appears to be mediated by increased rapid calcium buffering, which reduces the availability of calcium for uptake into the stores. An age-related decrease in the amplitude of maximal caffeine-induced calcium release was attributed to increased mitochondrial buffering. There were no age-related differences in the sensitivity to caffeine or in the calcium sequestration/release process at the level of the endoplasmic reticulum per se. These findings demonstrate the importance of interactions between cellular calcium buffering mechanisms and provide details regarding age-related changes in calcium homeostasis which have been thought to occur in these and other neurons associated with age-related neuronal dysfunctions.

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^☆: Correspondence to: W.H. Griffith, 307 Reynolds, Department of Medical Pharmacology, Texas A&M University, College Station, TX 77843–1114, USA. Fax: +1 409 845 0699; e-mail: [email protected]

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ResearchAge-related alterations in caffeine-sensitive calcium stores and mitochondrial buffering in rat basal forebrain☆

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Age-associated changes in Ca2+-dependent processes: relation to hippocampal synaptic plasticity

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Ion-transport systems and Ca2+regulation in aging neurons

NY Acad Sci

The septohippocampal pathway: structure and function of a central cholinergic system

Physiol Rev

Degenerative changes in forebrain cholinergic nuclei correlate with cognitive impairments in aged rats

Eur J Neurosci

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Low-voltage activated calcium currents increase in basal forebrain neurons from aged rats

J Neurophysiol

High-voltage activated calcium currents in basal forebrain neurons during aging

J Neurophysiol

Research
Age-related alterations in caffeine-sensitive calcium stores and mitochondrial buffering in rat basal forebrain☆

Age-associated changes in Ca²⁺-dependent processes: relation to hippocampal synaptic plasticity

Ion-transport systems and Ca²⁺regulation in aging neurons