Basic—Alimentary TractMicroRNAs Are Differentially Expressed in Ulcerative Colitis and Alter Expression of Macrophage Inflammatory Peptide-2α
Section snippets
Human Tissues
Colonoscopic pinch biopsies from the sigmoid colon of patients with chronic active UC, chronic inactive UC, chronic active colonic CD, IBS, IC, MC, and normal, healthy patients undergoing screening colonoscopies (Table 1) were obtained using a protocol approved by The Johns Hopkins University Institutional Review Board. In total, 62 patient biopsies were assessed. The diagnoses of active UC, inactive UC, MC (2 cases of collagenous colitis and 1 case of lymphocytic colitis), and CD were
miRNAs Are Differentially Expressed in UC Tissues
We first sought to determine whether miRNAs are differentially expressed in active UC.
Sigmoid colon pinch biopsies from patients with histologically confirmed active UC, inactive UC, and normal, healthy control subjects were obtained. Additional control groups used for comparison included patients with IBS, IC, MC, and CD. Clinical characteristics of each patient group are listed in Table 1.
An miRNA microarray capable of measuring the expression of 553 known human miRNA genes was used to
Discussion
In this study, we demonstrated that miRNAs are differentially expressed in the tissues of patients with active UC as compared with normal, healthy control subjects. Specifically, we identified 8 up-regulated and 3 down-regulated miRNAs in active UC tissues. The pattern of expression of these active UC-associated miRNAs was distinct from other conditions, including inactive UC, IC, MC, CD, and IBS. This study is the first to link chronic IBD with altered expression of miRNAs, thereby expanding
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Supported by National Institutes of Health grants K08DK078046 (to J.H.K.) and R24DK064388 and by Broad Medical Research Program grants IBD-0051 (to F.W. and S.C.) and IBD-0212 (F.W. and J.H.K.). J.H.K. was also supported by the Sherlock Hibbs IBD Research Fund, the M. Alan Guerrieri Family Fund and the Meyerhoff IBD Center at The Johns Hopkins University.