Microarray and other new technologyRecurrent Genomic Alterations With Impact on Survival in Colorectal Cancer Identified by Genome-Wide Array Comparative Genomic Hybridization
Section snippets
Study Materials
Surgical specimens from 59 CRC patients who underwent surgical resection from 1995 to 1997 at Dankook University Hospital in Cheonan, Korea, were examined in this study. This study was performed under the approval of the Institutional Review Boards of Kangnam St. Mary’s Hospital and The Catholic University of Korea. After surgical resection, tumor and adjacent normal tissues from each patient were collected separately and snap-frozen. Frozen sections were prepared of 10-μm thickness on a
General Characteristics of Study Subjects and Genomic Alterations in 59 CRCs
The clinicopathologic data of all 59 patients are summarized in Table 1. There were 39 men and 20 women and the mean patient age at the time of surgery was 58.7 years (range, 23–81 y). Among the 59 patients, 41 (69.5%) had rectosigmoid cancer. Thirty-six cancers (61.0%) were categorized as early stage tumors. At the end of the follow-up period, 23 patients were dead.
The overall genomic alterations detected in 59 colorectal cancers are illustrated in Figure 1A. The frequency plot of the
Discussion
CRC is one of the well-studied neoplastic diseases at the molecular level. A genetic model of colorectal tumorigenesis and related critical mutations such as APC, RAS, and p53 have been well postulated.4 However, clinically applicable biomarkers that reliably predict clinical outcome in individual CRC cases still are scarce. By using whole-genome array CGH in this study, we successfully identified novel chromosomal aberrations and a putative tumor-suppressor gene in a recurrently deleted
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2016, Cancer GeneticsCitation Excerpt :The data presented here indicate that combining CMA and NGS yields more actionable information about relevant changes in the tumor genome and may better guide treatment decisions than either technology alone. The largest exome sequencing study of lung and colon tumor genomes to date was published by the Cancer Genome Atlas (36,37). Consistent with this and numerous other studies (38–40), most of the significantly recurrent actionable genomic alterations detected by both DNA sequencing and CMA in the colon cohort were observed at known cancer-related genes, such as APC, TP53, KRAS, PIK3CA, FBXW7, SMAD4, APC, SMARCB1, and PTEN.
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Supported by the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (0405-BC02-0604-0004).
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M.-Y.K. and S.-H.Y. contributed equally to this paper.