Synthesis of 6-benzylaminopurine and the study of biological active properties of cotton C-6424 plants

. 6-Benzylaminopurine is a broad-spectrum plant growth regulator, able to rejuvenate plants, bring plants out of dormancy, cause the formation of lateral shoots and root shoots, stimulates the formation of chlorophyll and positively affects photosynthesis, making the leaf darker and greener, stronger, in many crops are able, with out-of-root processing of green fruits and berries, to somewhat postpone the ripening time in favor of size and weight. The variety of heterocyclic compounds is due to the possibility of variations: the number and nature of heteroatoms in a molecule, the ring size, the degree of unsaturation, which determines the presence or absence of aromaticity, and the possibility of the existence of condensed structures. The main attention in the methodological development is given to the most common methods of synthesis and the chemical properties of the main classes of aromatic heterocyclic structures. This choice is due to the fact that it is aromatic heterocycles that exhibit specific chemical properties inherent only to these classes of compounds, while the properties of saturated structures or unsaturated non-aromatic heterocycles, as a rule, are similar to their acyclic analogs.


Introduction
The chemistry of heterocycles is one of the most exciting and important areas of organic chemistry. Suffice it to say that more than 60% of the most well-known and widely used drugs of natural and synthetic origin are heterocyclic compounds. The presence of a heteroatom in the cycle introduces a unique originality into the chemical properties and determines the specifics of the synthesis methods. Information about the presence of heterocyclic compounds in natural objects, about drugs containing heterocycles and other "minor", in our opinion, information is in small print and is intended for the development of general erudition, and not for memorization and learning [1][2][3][4][5][6][7][8].
6-Benzylaminopurine also activates the synthesis of RNA and proteins, enhances transpiration, delays the aging process of plants and increases their resistance to adverse environmental conditions.
In plants, 6-benzylaminopurine is formed in the roots during the breakdown of transport RNAs, as well as by biosynthesis from 5'-adenosine monophosphate and isopentenyl pyrophosphate (the latter is obtained from mevalonic acid); with further enzymatic cleavage of the phosphate and riboside groups, iso-pentenyladenine is obtained, which is oxidized to zeatin.
In the form of transport forms-corresponding nucleosides and nucleotides, 6benzylaminopurine moves along the xylem to the aerial parts of plants. In plant tissues, 6benzylaminopurine decomposes quite quickly with the cleavage of the side chain and further with the rupture of the purine ring; more stable are their transport forms, as well as reserve conjugate forms that form with glucose, alanine, and some proteins by attaching them to ring N atoms or side chain atoms [9][10][11][12][13][14]. This scientific article is to study the synthesis of 6benzylaminopurine and the biological activity of the coordination compound formed by cobalt (II) nitrate hexahydrate, the effect of the cotton variety C-6243 on root growth, as well as the structure of the resulting complex compound by IR spectroscopy.

Materials and methods
Adenine, sodium benzylate and benzyl alcohol were added to the flask (the molar ratio of adenine, sodium benzylate, benzyl alcohol is 1:1; 8.7) and boiled for 2.5 hours with stirring. Cooled to room temperature, 150 ml diethyl ether was added and the precipitate was filtered. Received sodium salt of 6-benzylaminopurine, yield 94%.
Sodium salt of 6-benzylaminopurine was dissolved in 150-200 ml of hot water, 1.3-1.5 ml of acetic acid was added to pH 6.5-7.5, cooled to room temperature and filtered, dried. To synthesize the complex compound, a solution of Co(NO3)2 (1 mol) in 20 ml of ethanol was added dropwise to a solution of 6-benzylaminopurine (BAP) (2 mol) in 10 ml of alcohol. The resulting clear solution was refluxed with a magnetic stirrer at 80° C. for 50 minutes. Then the reaction mixture was cooled to room temperature. After five days, the formed long reddish-brown needle-like crystals were filtered off, washed several times with cold alcohol, recrystallized from boiling alcohol, and dried in the open air at room temperature.

Results and discussion
The effect of the reaction of the coordination compounds of cobalt II nitrate with 6benzylaminopurine of Gossypium cotton on varieties "C-6424" was studied.
Cotton seeds "S-6424" were used to assess the growth activity of the coordination compound of the synthesized substances. For laboratory experiments, concentrations of substances of 0.1% -0.001% were used, and the growth regulator radipharm was chosen as a reference. The expected results in our experiment consists of the following steps.
Coordination compounds of cobalt II nitrate with 6-benzylaminopurine we prepared an alcoholic solution of 0.1% and 0.001% solutions were prepared in distilled water. 20 seeds in a 0.1% solution for 5 and 10 hours, 20 seeds in a 0.001% solution for 5 and 10 hours were poured into the solution.
Influence of coordination compounds of cobalt II nitrate with 6-benzylaminopurine (BAP) on seed germination. Seeds 4 Petri dishes and 20 pieces at equal distances. In total, the sample was placed in a thermostat in 5 Petri dishes. Temperature 27 °C, humidity 40%. 2.5 and 5 hours, the seeds are soaked in a 0.1% solution for 10 hours. there were processes of increase, 0.1% for 5 hours, the seeds are soaked in a solution for 10 hours, processes of enlargement were observed. The root growth rate is performed in the same order. For 5 hours in a 0.01% solution as the roots grow. It is desirable to sow seeds evenly, we expect.
As can be seen from Table 1 and Figure 1, the coordination compound formed by BAP showed growth activity at a concentration of 0.1%, the root length increased by 43.1% compared with the control, the stem height by 59.6% and compared with the reference drug Radifarm, respectively. It was found that this synthesized substance increased the length of the root and stem of cotton by 43.1% and 59.6% compared with the control at a concentration of 0.1%. The results obtained are expressed in the form of a diagram ( Figure 2).
As can be seen from Figure 2, it was found that a 0.1% concentration of a solution of the agreed biologically active substance BAP, synthesized with cobalt nitrate 2, on the cotton variety "C-6424" had an effect on the growth of roots and stems. This is expressed in the lines of the diagram.
The structure of the resulting complex compounds was analyzed by IR spectroscopy ( Table 2).  According to the results of IR analysis (Figure 3), the following vibrational frequencies were observed in the IR spectrum of the BAP ligand: 1312 cm -1 (νC-N), 1443 cm -1 (νN=C). In the complex compound of cobalt-II nitrate hexahydrate, synthesized on the basis of the organic substance BAP, the following changes were observed. Due to the replacement of the nitrogen atom of the ligand by the hydrogen atom of the metal, stretching vibrations of the Me-N bond appeared, which were observed in the regions of 557 and 499 cm -1 in the doublet state. A change in the stretching vibrations of the νC=N group up to 526 cm -1 was found. In turn, when passing from the ligand to complex compounds, a change in the lines of stretching vibrations of the νC-N group was observed, that is, the vibration lines of this group split in two and changed their values to higher ones with an area of 78-30 cm -1 compared to the ligand.

Conclusions
Cobalt is necessary for plants to absorb molecular nitrogen, it is a trace element, legume nodes and the formation of nodular bacteria on the leaves will give. Cobalt accumulates in the wood of the plant and accelerates growth, participates in the metabolism of oxin, that is, an important nutrient for plant growth processes, including cell membranes, helps to lengthen. This metal ion is involved in the growth of leaf cells. Increase in thickness and volume of mesophilic, columnar and cell volume in cloudy leafy parenchyma and dormouse. In addition, cobalt is common water for plants increases the content and therefore the drought of crops increases longevity. The concentration of chloroplasts and pigments in the leaves, the formation of the photosynthetic apparatus of plants and the effect of cobalt coordination compound is very important.