The equilibrium geometry structures and light absorption properties of the dopamine (DA) and dopamine-o-quinone (DAQ) adsorbed on the graphene surface have been investigated using the ground state and linear-response time-dependent density functional theories. Two types of graphene systems were considered, a rectangular form of hexagonal lattice with optimized C–C bond length as the model system for graphene nanoparticles (GrNP) and a similar system but with fixed C–C bond length (1.42 Å) as the model system for graphene 2D sheet (GrS). The analysis of the vertical excitations showed that three types of electronic transitions are possible, namely, localized on graphene, localized on the DA or DAQ, and charge transfer (CT). In the case of the graphene–DA complex, the charge transfer excitations were characterized by the molecule-to-surface (MSCT) character, whereas the graphene–DAQ was characterized by the reverse, i.e. surface-to-molecule (SMCT). The difference between the two cases is given by the presence of an energetically low-lying unoccupied orbital (LUMO+1) that allows charge transfer from the surface to the molecule in the case of DAQ. However, it was also shown that the fingerprints of excited electronic states associated with the adsorbed molecules cannot be seen in the spectrum, as they are mostly suppressed by the characteristic spectral shape of graphene.