Volume 3, 2024
From the journal:

Sensors & Diagnostics

Acid-promoted fluorescent probe for monitoring endogenous methylglyoxal in tumors and gastritis†

Weijia Xu,‡a   Senyao Liu,‡a   Wenwen Caoa  and  Hu Xiong ORCID logo *a  

* Corresponding authors

a Research Center for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China
E-mail: xionghu@nankai.edu.cn

Abstract

Methylglyoxal (MGO) is an important dicarbonyl metabolite that plays a crucial role in a range of physiological and pathological processes. It is critical to develop precise methods for detecting MGO levels in vivo to better understand its role in disease progression. Herein, we report an MGO-activatable fluorescent probe Cy-DNH2 based on hemicyanine, which could rapidly and selectively detect MGO with a low LOD of 95 nM in an acidic microenvironment. In the presence of MGO, acidic pH could trigger Cy-DNH2 to turn on intense fluorescence within 3 min. Moreover, Cy-DNH2 was able to target lysosomes, allowing for the detection of endogenous MGO in living cells as well as the identification of elevated MGO levels in tumors and mouse models of gastritis. This work may provide a valuable tool for monitoring MGO under acidic conditions and diagnosing MGO-related diseases.

Graphical abstract: Acid-promoted fluorescent probe for monitoring endogenous methylglyoxal in tumors and gastritis

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Article information

DOI
https://doi.org/10.1039/D3SD00228D
Article type
Paper
Submitted
29 Aug 2023
Accepted
11 Nov 2023
First published
14 Nov 2023
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2024,3, 147-152

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Acid-promoted fluorescent probe for monitoring endogenous methylglyoxal in tumors and gastritis

W. Xu, S. Liu, W. Cao and H. Xiong, Sens. Diagn., 2024, 3, 147 DOI: 10.1039/D3SD00228D

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