Cytotoxicity assessment of exfoliated MoS2 using primary human mast cells and the progenitor cell-derived mast cell line LAD2

Hazel Lin; Antonio Esau del Rio Castillo; Viviana Jehová González; Francesco Bonaccorso; Ester Vázquez; Bengt Fadeel; Alberto Bianco

doi:10.1039/D3NA00863K

Cytotoxicity assessment of exfoliated MoS₂ using primary human mast cells and the progenitor cell-derived mast cell line LAD2†

Hazel Lin,^a Antonio Esau del Rio Castillo,^b Viviana Jehová González,

^c Francesco Bonaccorso,^b Ester Vázquez,

^c Bengt Fadeel

^d and Alberto Bianco

*^a

Author affiliations

* Corresponding authors

^a CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR 3572, University of Strasbourg, ISIS, 67000 Strasbourg, France
E-mail: a.bianco@ibmc-cnrs.unistra.fr

^b BeDimensional, Lungo Torrente Secca 30r, Genoa, Italy

^c Biograph Solutions, Regional Institute of Applied Scientific Research (IRICA), Department of Organic Chemistry, Faculty of Science and Chemistry Technologies, University of Castilla-La Mancha, Ciudad Real 13071, Spain

^d Nanosafety & Nanomedicine Laboratory, Institute of Environmental Medicine, Karolinska Institutet, 177 77 Stockholm, Sweden

Abstract

Molybdenum disulfide is an emerging 2D material with several potential applications in medicine. Therefore, it is crucial to ascertain its biocompatibility. Mast cells are immune cells that are found in many organs and tissues in contact with the extracellular environment, and can be cultured from progenitor cells present in the bone marrow. Given the long period required for differentiation and proliferation of primary mast cells, human mast cell lines have emerged as a tractable model for biological and toxicological studies. Here, we compare two types of industrial MoS₂ using CD34⁺-derived primary human mast cells and the LAD2 cell line. Minimal effects were observed on early-stage activation endpoints such as β-hexosaminidase release and expression of surface markers of mast cell activation. Transmission electron microscopy revealed limited uptake of the tested materials. Overall, MoS₂ was found to be biocompatible, and the LAD2 cell line was validated as a useful in vitro model of mast cells.

This article is part of the themed collection: Graphene and 2D Materials in Healthcare

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Article information

DOI: https://doi.org/10.1039/D3NA00863K
Article type: Paper
Submitted: 07 Oct 2023
Accepted: 27 Mar 2024
First published: 29 Mar 2024
This article is Open Access

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Nanoscale Adv., 2024,6, 2419-2430

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Cytotoxicity assessment of exfoliated MoS₂ using primary human mast cells and the progenitor cell-derived mast cell line LAD2

H. Lin, A. E. del Rio Castillo, V. J. González, F. Bonaccorso, E. Vázquez, B. Fadeel and A. Bianco, Nanoscale Adv., 2024, 6, 2419 DOI: 10.1039/D3NA00863K

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