Structural characterisation of deer sinew peptides as calcium carriers, their promotion of MC3T3-E1 cell proliferation and their effect on bone deposition in mice

Li Sun; Jinze Liu; Hongyan Pei; Meiling Shi; Weijia Chen; Ying Zong; Yan Zhao; Jianming Li; Rui Du; Zhongmei He

doi:10.1039/D3FO04627C

Structural characterisation of deer sinew peptides as calcium carriers, their promotion of MC3T3-E1 cell proliferation and their effect on bone deposition in mice†

Li Sun,

^a Jinze Liu,^a Hongyan Pei,

^a Meiling Shi,^a Weijia Chen,^a Ying Zong,^a Yan Zhao,^a Jianming Li,^a Rui Du

*^ab and Zhongmei He

*^ab

Author affiliations

* Corresponding authors

^a College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China

^b Jilin Provincial Engineering Research Centre for Efficient Breeding and Product Development of Sika Deer, Changchun 130118, China
E-mail: durui@jlau.edu.cn

Abstract

Deer sinew as a by-product has high collagen and nutritional value. This study focuses on its hydrolysate being used as a calcium carrier to develop functional foods. The chelation mechanism was analyzed by SEM, EDS, UV-vis, FTIR, and fluorescence spectroscopy and zeta potential analysis after using peptide-sequenced deer sinew peptides for chelation with calcium ions. The results showed that the chelation of deer sinew peptides with calcium ions occurs mainly at the O and N atoms of carboxyl, amino and amide bonds. In vitro and in vivo studies revealed that deer sinew peptide–calcium chelate (DSPs-Ca) promoted the proliferation of MC3T3-E1 cells without toxic side effects and increased the alkaline phosphatase activity. The DSPs-Ca group improved the bone microstructure induced by low calcium, as well as up-regulated the expression of genes responsible for calcium uptake in the kidneys, as evidenced by serum markers, bone sections, bone parameters, and gene expression analyses in low-calcium-fed mice. From the above, it can be concluded that DSPs-Ca is expected to be a calcium supplement food for promoting bone health.

Food & Function

Structural characterisation of deer sinew peptides as calcium carriers, their promotion of MC3T3-E1 cell proliferation and their effect on bone deposition in mice†

Abstract

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Structural characterisation of deer sinew peptides as calcium carriers, their promotion of MC3T3-E1 cell proliferation and their effect on bone deposition in mice

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