A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes

Liqun Dai; Siming Zhou; Cheng Yang; Jie Li; Yayue Wang; Meng Qin; Lili Pan; Dan Zhang; Zhiyong Qian; Haoxing Wu

doi:10.1039/D3CC05604J

A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes†

Liqun Dai,‡^ab Siming Zhou,‡^a Cheng Yang,^a Jie Li,^c Yayue Wang,

^c Meng Qin,^d Lili Pan,^e Dan Zhang,*^a Zhiyong Qian*^b and Haoxing Wu

*^ac

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* Corresponding authors

^a Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
E-mail: danzhang@scu.edu.cn, haoxingwu@scu.edu.cn

^b Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China
E-mail: nderson-qian@163.com

^c Department of Radiology and Huaxi MR Research Center (HMRRC), Functional and Molecular Imaging Key Laboratory of Sichuan Province and Frontiers Science Center for Disease Related Molecular Network West China Hospital, Sichuan University, Chengdu 610041, China

^d National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China

^e Department of Nuclear Medicine, Laboratory of Clinical Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China

Abstract

Here we describe a cost-effective and simplified cell sorting method using tetrazine bioorthogonal chemistry. We successfully isolated SKOV3 cells from complex mixtures, demonstrating efficacy in separating mouse lymphocytes expressing interferon and HeLa cells expressing virally transduced green fluorescent protein post-infection.

Chemical Communications

A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes†

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A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes

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