A ratiometric fluorescent probe with a large Stokes shift for the detection of Hg2+ and its applications in environmental sample and living system analysis

Jixiang Tian; Xuechun Tian; Shuai Gong; Yueyin Liang; Zhiyuan Meng; Weiqi Liu; Xu Xu; Zhonglong Wang; Shifa Wang

doi:10.1039/D3AY02106H

A ratiometric fluorescent probe with a large Stokes shift for the detection of Hg²⁺ and its applications in environmental sample and living system analysis†

Jixiang Tian,^a Xuechun Tian,^a Shuai Gong,^a Yueyin Liang,^a Zhiyuan Meng,^a Weiqi Liu,^a Xu Xu,

^a Zhonglong Wang*^a and Shifa Wang

*^a

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* Corresponding authors

^a Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
E-mail: wang_zhonglong@njfu.edu.cn, wangshifa65@163.com
Fax: +86-25-85428369
Tel: +86-25-85428369

Abstract

Toxic mercury ions (Hg²⁺) can cause serious environmental pollution and accumulate in living organisms via the food chain. Therefore, monitoring Hg²⁺ is crucial in ensuring the safety of ecosystems and organisms. In this work, a novel ratiometric fluorescent probe CMT (5-(4-(diphenylamino)phenyl)-1-(7-hydroxy-coumarin-3-yl)-4-pentene-1,3-dione) based on coumarin was developed for detecting Hg²⁺, which displayed obvious fluorescence changes, a low detection limit (2.24 × 10⁻⁷ M), good selectivity, and a large Stokes shift (255 nm). The CMT probe could detect Hg²⁺ in real environmental soil and water samples. Furthermore, the CMT probe enabled the naked-eye detection of Hg²⁺ using test paper experiments. CMT was also applied for fluorescence imaging in living zebrafish and plants. This work provides a highly efficient tool for monitoring Hg²⁺ in environmental samples and biological systems.

This article is part of the themed collection: Analytical Methods HOT Articles 2024

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Article information

DOI: https://doi.org/10.1039/D3AY02106H
Article type: Paper
Submitted: 26 Nov 2023
Accepted: 04 Mar 2024
First published: 05 Mar 2024

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Anal. Methods, 2024,16, 1846-1855

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A ratiometric fluorescent probe with a large Stokes shift for the detection of Hg²⁺ and its applications in environmental sample and living system analysis

J. Tian, X. Tian, S. Gong, Y. Liang, Z. Meng, W. Liu, X. Xu, Z. Wang and S. Wang, Anal. Methods, 2024, 16, 1846 DOI: 10.1039/D3AY02106H

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Analytical Methods