Magnetic mesoporous silica nanoparticles modified by phosphonate functionalized ionic liquid for selective enrichment of phosphopeptides

Yufei Jiang; Weida Liang; Binbin Wang; Quanshou Feng; Chenglong Xia; Qiyao Wang; Yinghua Yan; Lingling Zhao; Wei Cui; Hongze Liang

doi:10.1039/D2RA04609A

Magnetic mesoporous silica nanoparticles modified by phosphonate functionalized ionic liquid for selective enrichment of phosphopeptides†

Yufei Jiang,^a Weida Liang,^a Binbin Wang,^a Quanshou Feng,^a Chenglong Xia,^a Qiyao Wang,^b Yinghua Yan,

^a Lingling Zhao,

^a Wei Cui*^b and Hongze Liang

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China
E-mail: lianghongze@nbu.edu.cn

^b Ningbo Key Laboratory of Behaviour Neuroscience, Zhejiang Province Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo 315211, China
E-mail: cuiwei@nbu.edu.cn

Abstract

In this study, new magnetic nanoparticles (denotated as Fe₃O₄@mSiO₂-PFIL-Ti⁴⁺) have been prepared by immobilizing titanium ions with phosphonate functionalized ionic liquid (PFIL) on the wall of core–shell structured mesoporous nanomaterials. The resulting nanoparticles possess large specific surface area, strong hydrophilicity and fast magnetic response. The composites can capture traces of phosphopeptides from the tryptic β-casein digest (0.08 fmol), a digest mixture of β-casein and BSA (1 : 10 000, molar ratio) as well as a blend of β-casein digest and a great quantity of phosphorylated protein (β-casein) and non-phosphorylated protein (BSA) (1 : 2000 : 2000, mass ratio), respectively, showing excellent sensitivity, selectivity and size exclusion ability. Additionally, Fe₃O₄@mSiO₂-PFIL-Ti⁴⁺ shows excellent steadiness and can be reused at least 12 times. Moreover, this material was successfully applied to enrich endogenous phosphopeptides from complex bio-samples, including human saliva and serum.

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Magnetic mesoporous silica nanoparticles modified by phosphonate functionalized ionic liquid for selective enrichment of phosphopeptides†

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