Issue 34, 2023
From the journal:

Chemical Communications

Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome†

Changjiang Fan, ORCID logo *ab   Xinyue Meng,ab   Wei Yang,ab   Peiyan Wang,ab   Wenguang Chang,a   Peifeng Li*a  and  Jianxun Wang*ab  

* Corresponding authors

a Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao 266021, Shandong, P. R. China
E-mail: cjfan@qdu.edu.cn, peifli@hotmail.com

b School of Basic Medicine, College of Medicine, Qingdao University, Qingdao 266071, Shandong, P. R. China
E-mail: wangjx@qdu.edu.cn

Abstract

A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation of a cleavable linker via the click reaction, achieving 8-oxoGua pull-down and sequencing. Using APSC-8-oxoGua-seq, 8-oxoGua can be identified at single-base resolution.

Graphical abstract: Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome

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Article information

DOI
https://doi.org/10.1039/D2CC06273A
Article type
Communication
Submitted
23 Nov 2022
Accepted
02 Apr 2023
First published
03 Apr 2023

Chem. Commun., 2023,59, 5106-5109

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Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome

C. Fan, X. Meng, W. Yang, P. Wang, W. Chang, P. Li and J. Wang, Chem. Commun., 2023, 59, 5106 DOI: 10.1039/D2CC06273A

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