Issue 72, 2022
From the journal:

Chemical Communications

Branched immunochip-integrated pairwise barcoding amplification exploring the spatial proximity of two post-translational modifications in distinct cell subpopulations†

Jing Xue,a   Youlan Fu,a   Siyue Fan,a   Xiaowen Cao,a   Wei Huang,a   Jin Zhang,a   Yongxi Zhao ORCID logo a  and  Feng Chen ORCID logo *a  

* Corresponding authors

a Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China
E-mail: fengchencf@mail.xjtu.edu.cn

Abstract

Investigating the spatial information of post-translational modifications (PTMs) in distinct cell subpopulations represents a new direction toward single-cell analysis. The specific capture of cell populations combined with PTM spatial proximity visualization making it practically challenging. Here, we develop branched immunochip-integrated pairwise barcoding amplification, termed biChip-PBA, which can perform the respective capture of cell subpopulations expressing different membrane proteins and successive PBA-based fluorescence imaging of PTM proximities. Our work may provide multilevel information for new insights into epigenetic regulation and cell function.

Graphical abstract: Branched immunochip-integrated pairwise barcoding amplification exploring the spatial proximity of two post-translational modifications in distinct cell subpopulations

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Article information

DOI
https://doi.org/10.1039/D2CC03833A
Article type
Communication
Submitted
09 Jul 2022
Accepted
11 Aug 2022
First published
11 Aug 2022

Chem. Commun., 2022,58, 10020-10023

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Branched immunochip-integrated pairwise barcoding amplification exploring the spatial proximity of two post-translational modifications in distinct cell subpopulations

J. Xue, Y. Fu, S. Fan, X. Cao, W. Huang, J. Zhang, Y. Zhao and F. Chen, Chem. Commun., 2022, 58, 10020 DOI: 10.1039/D2CC03833A

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