Amyloid-like Prep1 peptides exhibit reversible blue-green-red fluorescence in vitro and in living cells†
Luigi
Vitagliano 
*a
and
Nunzianna
Doti
*a
*a
and
Nunzianna
Doti
*a
Abstract
PREP1-based peptides form amyloid-like aggregates endowed with an intrinsic blue-green-red fluorescence with an unusual sharp maximum at 520 nm upon excitation with visible light under physiological conditions. The peptide PREP1[117-132], whose sequence does not contain aromatic residues, presents a pH-dependent and reversible fluorescence, in line with its structural transition from β-sheet rich aggregates to α-helix structures. These findings further demonstrate that the non-canonical fluorescence exhibited by amyloids is an articulated phenomenon.
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