Issue 6, 2021
From the journal:

RSC Advances

Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles

Zhen Lin, ORCID logo †a   Xiaohui Tan,†a   Fangping Li,†a   Yu Zhang,a   Ping Luo,a   Xuan Lina  and  Huazhong Liu*a  

* Corresponding authors

a College of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang 524088, China
E-mail: liuhzbs@163.com
Fax: +86-759-2383300
Tel: +86-759-2383300

Abstract

To explore the interactive molecules of squid ink polysaccharides (SIP) for further understanding the action mechanisms of SIP bio-function, this study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads. Michaelis–Menten constant (Km) was detected from a Lineweaver–Burk double reciprocal plot to assess effect of SIP on activity of aldehyde oxidase (AOX). Results showed that three proteins, AOX-3, regucalcin (RGN) and α1-antitrypsin (A1AT3) were separated from mouse liver by magnetic nanoparticles conjugated with SIP. Contents of AOX-3 were much more than RGN and A1AT3. SIP (0.5 mg mL−1) reduced Km value of aldehyde oxidase of mouse liver from 91.79 μmol L−1 to 43.70 μmol L−1.

Graphical abstract: Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles

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Article information

DOI
https://doi.org/10.1039/D0RA09222C
Article type
Paper
Submitted
29 Oct 2020
Accepted
06 Jan 2021
First published
18 Jan 2021
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2021,11, 3596-3602

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Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles

Z. Lin, X. Tan, F. Li, Y. Zhang, P. Luo, X. Lin and H. Liu, RSC Adv., 2021, 11, 3596 DOI: 10.1039/D0RA09222C

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