Issue 49, 2020
From the journal:

RSC Advances

Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration†

Xusheng Chen, ORCID logo *a   Wenjiao Diao,a   Yu Maa  and  Zhonggui Maoa  

* Corresponding authors

a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
E-mail: chenxs@jiangnan.edu.cn
Fax: +86 510 85918296
Tel: +86 510 85918296

Abstract

ε-Poly-L-lysine (ε-PL) serves as a natural food preservative and is manufactured mainly by extraction from microbial fermentation broth using ion-exchange chromatography. In order to develop an alternative purification strategy, an environmentally friendly alcohol/salt aqueous two-phase system (ATPS) was explored in this study for ε-PL extraction. A study of the separation of ε-PL in different alcohol/salt systems showed that ethanol/ammonium sulfate ATPS exhibited the highest ε-PL partition coefficient and recovery ratio. Based on the phase diagram, the effect of phase composition on partition, and the removal of pigment and protein, an ATPS that was composed of 20% (w/w) ethanol and 20% (w/w) ammonium sulfate, with a feedstock at pH 9.5, was developed to extract ε-PL from the fermentation broth. This achieved an ε-PL recovery ratio of 96.15% with an ε-PL purity of 40.23% after triplicate extractions. Subsequently, desalting by ultrafiltration led to a final ε-PL product of 92.39% purity and 87.72% recovery. The ethanol/ammonium sulfate ATPS provides a new possibility for ε-PL purification.

Graphical abstract: Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration

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Article information

DOI
https://doi.org/10.1039/D0RA04245E
Article type
Paper
Submitted
12 May 2020
Accepted
27 Jul 2020
First published
10 Aug 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 29587-29593

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Extraction and purification of ε-poly-L-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration

X. Chen, W. Diao, Y. Ma and Z. Mao, RSC Adv., 2020, 10, 29587 DOI: 10.1039/D0RA04245E

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