Issue 37, 2020

Double modulation SRS and SREF microscopy: signal contributions under pre-resonance conditions

Abstract

Pre-electronic resonance enhancement can increase the sensitivity of non-linear Raman microscopy to the single molecule detection limit. A major problem, however, is the generation of background signal due to unwanted linear and non-linear photophysical processes. In this work, we report the setup of a novel detection scheme for stimulated Raman scattering microspectroscopy based on the simultaneous modulation of pump and Stokes beam. Apart from allowing the parallel detection of stimulated Raman loss and gain (SRL and SRG), the setup gives access to the quantitative analysis of different sources of background signal. We report spectrally and temporally resolved measurements on three exemplary rhodamine dyes and derive the contributions of two-photon absorption and stimulated emission to their SRL, SRG, and stimulated Raman excited fluorescence signals. These results give guidelines for the further improvement of the sensitivity of non-linear Raman micospectroscopy under electronic pre-resonance conditions.

Graphical abstract: Double modulation SRS and SREF microscopy: signal contributions under pre-resonance conditions

Article information

Article type
Paper
Submitted
15 Jun 2020
Accepted
07 Sep 2020
First published
07 Sep 2020

Phys. Chem. Chem. Phys., 2020,22, 21421-21427

Double modulation SRS and SREF microscopy: signal contributions under pre-resonance conditions

P. Fimpel, A. J. X. Choorakuttil, A. Pruccoli, L. Ebner, S. Tanaka, Y. Ozeki, M. J. Winterhalder and A. Zumbusch, Phys. Chem. Chem. Phys., 2020, 22, 21421 DOI: 10.1039/D0CP03221B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements