Issue 40, 2020

A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity

Abstract

A triplex-forming oligonucleotide (TFO) linear probe containing perylene derivatives was synthesized. The TFO linear probe formed a remarkably stable triplex with a target DNA duplex, resulting in the light-up of fluorescence emission. The sensitivity was extremely high even at pH 7. Detection of PCR-amplified target DNA was demonstrated.

Graphical abstract: A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity

Supplementary files

Article information

Article type
Communication
Submitted
11 Mar 2020
Accepted
17 Apr 2020
First published
17 Apr 2020

Chem. Commun., 2020,56, 5358-5361

Author version available

A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity

Y. Chen, K. Murayama, H. Kashida, Y. Kamiya and H. Asanuma, Chem. Commun., 2020, 56, 5358 DOI: 10.1039/D0CC01865A

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