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A triple action chemosensor for Cu2+ by chromogenic, Cr3+ by fluorogenic and CN− by relay recognition methods with bio-imaging of HeLa cells

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Abstract

A triple action chemosensor (R1) bearing a rhodamine and thiophene moiety was synthesized by a simple condensation reaction. The sensing behaviour and selectivity of the synthesized chemosensor toward metal ions were studied by UV-Vis and fluorescence spectroscopy. The chemosensor recognized Cu2+ and Cr3+ ions with significant changes in UV-Vis absorbance and fluorescence intensity. The results showed that Cr3+ induced greater fluorescence enhancement whereas Cu2+ ions bound strongly with the receptor by showing a strong absorption band at 554 nm but with weak fluorescence. A visible colour change was observed by the addition of Cu2+ and that colour change is due to the opening of the spirolactam ring triggered by the addition of Cu2+ ions. Job’s plot analysis indicated a 1: 2 and 1: 1 binding stoichiometry between the chemosensor and Cr3+/Cu2+. Subsequently, the R1 − Cu2+ complex chemosensor was employed to detect CN− in the presence of different anions, such as F−, Cl−, Br−, I−, AcO−, H2PO4−, HSO4−, NO3− and −OH. In addition, the live cell imaging of HeLa cells using R1 and Cr3+ was demonstrated successfully.

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Acknowledgments

The authors thank the Director-NITT for support.

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Authors and Affiliations

  1. Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli, 620 015, India

    Natarajan Vijay, Gopal Balamurugan & Sivan Velmathi

  2. Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300

    Parthiban Venkatesan & Shu Pao Wu

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  1. Natarajan Vijay
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  2. Gopal Balamurugan
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  3. Parthiban Venkatesan
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  4. Shu Pao Wu
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  5. Sivan Velmathi
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Correspondence to Natarajan Vijay.

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Vijay, N., Balamurugan, G., Venkatesan, P. et al. A triple action chemosensor for Cu2+ by chromogenic, Cr3+ by fluorogenic and CN− by relay recognition methods with bio-imaging of HeLa cells. Photochem Photobiol Sci 16, 1441–1448 (2017). https://doi.org/10.1039/c7pp00163k

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