Deacetylation of mycothiol-derived ‘waste product’ triggers the last biosynthetic steps of lincosamide antibiotics† †Electronic supplementary information (ESI) available: Experimental procedures, additional figures, chromatograms and NMR data. See DOI: 10.1039/c5sc03327f Click here for additional data file.

Two homologous pyridoxal 5′-phosphate-dependent enzymes LmbF and CcbF transform the deacetylated S-cysteinyl residue of related intermediates in the biosynthesis of lincomycin/celesticetin in different ways.


Introduction
Zhao et al. recently discovered the intriguing participation of two low-molecular-weight thiols, mycothiol (MSH) and ergothioneine, in the biosynthesis of the lincosamide antibiotic, lincomycin A (hereinaer lincomycin or LIN; Fig. 1). 1 Such unusual involvement of these actinomycete thiols (Fig. 2B) in biosynthesis had remained undiscovered for a long time, mainly because the sulfur atom of MSH remains the only footprint of the thiols in the lincomycin structure. Ergothioneine is, however, required for condensation of the amino acid and amino octose unit to obtain the scaffold of lincomycin, whereas MSH plays its role immediately aer the condensation. In the condensation process, the stand-alone adenylation domain LmbC catalyses 4-propyl-L-proline activation and transfer on the holo-form of the carrier protein domain of LmbN, 2,3 whereas LmbT catalyses the formation of the octose conjugate with ergothioneine. 1 This conjugate is then condensed with the activated 4-propyl-L-proline via an amide bond in a reaction catalysed by LmbD ( Fig. 2A-C). 1 Interestingly, the following post-condensation steps are at least formally reminiscent of the MSH-dependent detoxication system present in actinomycetes, which is generally used for the elimination of electrophilic toxins, including various antibiotics and their metabolites, from the cell. 4 Following this parallel, ergothioneine is from the S-conjugated condensation product replaced for MSH by nucleophilic substitution in a reaction catalysed by LmbV. 1 The resulting product corresponds to a 'toxin'-MSH conjugate, where the lincomycin scaffold formally represents the 'toxin'.
Subsequently, LmbE cleaves off the pseudo-disaccharide 1-O-glucosamine-D-myo-inositol, resulting in compound 507, a mercapturic acid derivative characterized by the N 0 -acetyl-Scysteine residue, the only remainder of MSH in its structure ( Fig. 2C). 1 The structure of 507 corresponds to the 'waste product' of the MSH-dependent detoxication system, where mercapturic acid derivatives are generally predetermined for transport out of the cell. 4,5 Thus, the N 0 -acetyl-S-cysteinyl of the mercapturic acid derivative may be considered as a 'detoxication label'. Interestingly, the seeming 'waste product' 507 is further matured to give the antibiotic product lincomycin. When comparing the structures of 507 and lincomycin, it is obvious that the N 0 -acetyl-S-cysteinyl 'detoxication label' of 507 has to be removed and that two methylations at the nitrogen and sulfur atoms have to occur to give rise to lincomycin. The Nmethylation has been demonstrated to be catalysed by LmbJ in the biosynthesis of lincomycin and its homologue CcbJ in the biosynthesis of the related lincosamide, celesticetin (Fig. 1). 6 Moreover, the crystal structure of the homohexamer CcbJ has been solved, and the detailed reaction mechanism has been proposed. 7 However, the mechanism that switches the machinery from the 'detoxication' mode to the biosynthetic mode and how the nal biosynthetic steps proceed remains elusive.
Here, we demonstrate that the acetyl group of the N 0 -acetyl-Scysteinyl 'detoxication label' represents a locked door for further maturation of the 'waste product' 507 into the antibiotic lincomycin. Furthermore, we present the complete processing of the 'detoxication label' so that only its sulfur atom remains in the structure of lincomycin. In addition, we discuss apparent differences in the nal steps of lincomycin and celesticetin biosynthesis.

Results and discussion
New intermediates of lincomycin biosynthesis reveal conversion of 'waste product' 507 to antibiotic lincomycin We have detected four intermediates of lincomycin biosynthesis in the culture broths of two mutant strains of the lincomycin producer, Streptomyces lincolnensis, with specic genes deleted (see Experimental in ESI †). Apart from the known intermediate 507 (Fig. 2C), three new hypothetical intermediates, 465, 479 (Fig. 2D) and 521, were found (compounds are named according to their nominal mass). The compounds mutually differ in the presence of the N-methyl group in their structures (present only in 479 and 521) and in the presence of the intact N 0 -acetyl-Scysteinyl 'detoxication label' (present only in 507 and 521, whereas 465 and 479 have the 'detoxication label' deacetylated); see the graphic cartoon in Fig. 3 for the structural differences. We identied the compounds based on high resolution mass spectrometry (MS) and in-source collision-induced dissociation MS fragmentation and puried them from culture broths (Fig. S1 †). The structures were further elucidated by NMR analysis (NMR data in Table S2, † 1 H and 13 C NMR spectra in Fig. S2 †) and by incorporation experiments with Lcysteine-13 C 3 , 15 N, which conrmed that L-cysteine is part of the molecular structure of the four intermediates (details in Fig. S3 †).
The compounds were then tested in vitro as substrates of the recombinant proteins LmbJ and LmbF to interrogate the pathway from 507 to lincomycin (the purity and integrity can be assessed from SDS-PAGE gels in Fig. S4 †). The cartoon in Fig. 3 summarizes the results presented and is discussed in more detail below. Specically, we demonstrate that the main biosynthetic route proceeds as follows: rst, compound 507, the proven product of the LmbE reaction, 1 is deacetylated by a yet unassigned enzyme to yield 465, then N-methylated by LmbJ to yield 479, which is subsequently converted into 392 by LmbF and nally S-methylated, presumably by LmbG, to give lincomycin. Additionally, we show that the relaxed substrate speci-cities of biosynthetic proteins enable either documented alternative or hypothetical bypasses (see Fig. 2D for the main stream; cartoon in Fig. 3 for all possible streams).

Ornamentation by LmbJ N-methyltransferase is not the last biosynthetic step
In previous work by Najmanova et al., 6 it has been shown that LmbJ N-methylates the chemically synthesized N-demethyllincomycin (NDL), suggesting that N-methylation of the 4propyl-L-proline moiety is the last step in lincomycin biosynthesis. However, the existence of the biosynthesized N-methylated intermediates 521 and 479 raises questions about whether the N-methylation occurs at an earlier stage. Indeed, we tested recombinant LmbJ with 507, 465 and NDL and revealed that all of these substrates can be converted into the N-methylated products 521, 479 and lincomycin, respectively (see Fig. 3 for the scheme and Fig. S5 † for the LC-MS data). However, the rapid and preferential conversion of 465 but not 507 or NDL in a competitive in vitro assay strongly suggests that 465 is the main natural substrate of LmbJ (Fig. 4). This nding also provides an explanation of the previously observed similar kinetic parameters of the homologous N-methyltransferases LmbJ and CcbJ for the conversion of NDL into lincomycin: 6 NDL is not the natural substrate for any of these two proteins. Moreover, these results conrm the broad substrate specicity of LmbJ, as predicted. 7 'Detoxication label' is removed by LmbF only if the 'label' is rst deacetylated The hypothetical lincomycin intermediates 465 and 479 have their 'detoxication label' deacetylated, suggesting that deacetylation is involved in lincomycin maturation. Because we detected 479 as the major intermediate in the mutant strain of S. lincolnensis with an inactive lmbF gene, we considered that LmbF played a role in the subsequent conversion. Indeed, we detected signicant consumption of 479 in the in vitro assay with recombinant LmbF (Fig. 5). The sequence analysis of LmbF shows that it belongs to the AAT_I (aspartate aminotransferase, fold type I) superfamily of enzymes, which employs pyridoxal-5 0phosphate (PLP) as a cofactor. Since a typical reaction for PLPmediated turnovers is transamination (and with respect to celesticetin biosynthesis, as explained in the later text), we expected that LmbF would replace the primary amino group of 479 with the oxo functional group. Surprisingly, we did not detect a product corresponding to a simple transamination reaction. Instead, we found compound 392 (see Fig. 2D and 3) to be the product, which means that LmbF is responsible for the removal of the whole S-cysteine residue, i.e., the complete deacetylated 'detoxication label' except for the sulfur atom (see LC-MS data in Fig. 5 and S6 †). LmbF failed to carry out this  Equimolar amounts of substrates were tested simultaneously in one reaction; n ¼ 3. Apeak area of the product, A ipeak area of the internal standard. reaction in the absence of PLP (Fig. S7 †), conrming that the cofactor is essential for the turnover. Further, we observed that LmbF is also capable of removing the S-cysteine residue from 465 to give 378, but less readily than from 479 ( Fig. S6 and S7 †), indicating that 479 is the main natural substrate of LmbF (this is also supported by the predominant presence of 479 over 465 in the culture broth of the strain with the inactive lmbF gene depicted in Fig. S8 †). On the other hand, we did not detect any turnover of 507 or 521 (LC-MS data in Fig. 5 and S7A †) where the primary amino group is not available due to its acetylation.
These ndings provide insight into the reaction mechanism of the 'detoxication label' processing aer it has been 'unlocked' by the deacetylation (Fig. 6): the unblocked primary amino group of 479 binds to PLP (a cofactor of LmbF) and LmbF catalyses the cleavage of the C-S bond by the b-elimination mechanism, which was described for PLP-dependent enzymes previously. 12 This results in the release of 392 and pyruvate, which was also detected as a product of the reaction (Fig. 5).

Reactive sulydryl group is protected by methylation
LmbF products 378 and 392 contain the exposed sulydryl group, which is prone to oxidation. Indeed, we observed a signicant decrease of 378 and 392 along with the formation of dimers of these compounds (Fig. S9 †) when the reaction time was prolonged from 2 h to 24 h. Therefore, we assume that the methylation of the sulydryl group occurs immediately aer the removal of the S-cysteine residue to protect this reactive group. This reaction has been proposed to be catalysed by LmbG 3 because it represents the only putative methyltransferase encoded within the lincomycin gene cluster whose function has not been assigned yet. The remaining two methyltransferases LmbW and LmbJ were proved to act as a C-methyltransferase 13 and a N-methyltransferase, 6 respectively, modifying the lincomycin amino acid unit.
'Detoxication label' abolishes antibacterial activity Furthermore, we tested whether the intermediates 507, 521, 465 and 479 exhibit antibacterial activity against the lincomycinsensitive Gram-positive bacterium Kocuria rhizophila and we compared the results with those for NDL and lincomycin. None of the intermediates containing the complete or deacetylated 'detoxication label' exhibited antibacterial properties against K. rhizophila (Fig. S10 †). A comparison of the results for NDL and lincomycin shows that the N-methyl group enhances the antimicrobial activity; however, it is not crucial. On the other hand, the presence of the 'detoxication label' completely abolishes the antibiotic activity of the compound. This observation is in accordance with previous studies indicating that mercapturic acid derivatives are less bioactive compared to the parent antibiotic. 14 Furthermore, not even deacetylation of the 'detoxication label' (intermediates 465 and 479) is a sufficient condition for antimicrobial activity.
Lincomycin/celesticetin biosynthesis: homologous LmbF/ CcbF catalyse a different type of reaction, forming a branch point in the related pathways Lincomycin and celesticetin ( Fig. 1) are the only known natural lincosamide antibiotics encoded by two independent but related biosynthetic gene clusters. 3,15 In contrast to lincomycin, with its methylated sulfur atom, celesticetin bears salicylate attached to sulfur via a two-carbon (2C) linker. Particularly, the origin of the 2C linker was unclear until the lincomycin intermediate 507 was revealed. We assume that condensation of celesticetin proceeds through a mechanism analogous to that of lincomycin, via a hypothetical intermediate 451 (Fig. 7), which would be analogous to 479; both compounds should bear the same deacetylated 'detoxication label'. However, the fate of the 'detoxication label' is probably different in the biosynthesis of celesticetin and lincomycin (see the proposed scheme in Fig. 7). Based on the celesticetin structure, it can be assumed that the 2C linker remains from the S-cysteine residue. Indeed, we have conrmed that these two carbons originate from L-cysteine since we achieved 78% incorporation of two 13 C atoms into the celesticetin 2C linker when the culture medium was supplemented with L-cysteine-13 C 3 , 15 N ( Fig. 7 and S11 †). This means that CcbF does not catalyse the cleavage of the C-S bond by the b-elimination mechanism. Instead, CcbF presumably performs decarboxylation-dependent transamination, a reaction known  to be catalysed by PLP-dependent enzymes as reviewed by John 16 and documented on dialkylglycine decarboxylase, for example. 17 The amino group would be replaced by an oxo group, which can be further reduced to a hydroxyl group (putative oxido-reductase encoded by the ccb5 gene in the celesticetin gene cluster would be a suitable candidate for this reductase activity). Having completed this course of reactions, the intermediate is ready for conjugation with the acyl unit (already completed salicylate or its precursor) to form the nal celesticetin scaffold (putative acyltransferase Ccb1, putative salicyl-AMP ligase Ccb2 and putative salicyl synthase Ccb3 can be expected to catalyse the formation and attachment of salicylate).

Conclusion and perspectives
In this work, we present the nal steps of lincomycin biosynthesis leading from the last proven intermediate, 507, to lincomycin. Interestingly, we have demonstrated that LmbJ Nmethylates a different substrate at an earlier stage of the biosynthesis than previously suggested. Furthermore, we have revealed that deacetylation of the 'waste product' 507 represents the key step that switches the detoxication-like MSH-dependent process towards the biosynthetic mode. Deacetylation provides a free primary amino group for the PLP-dependent LmbF responsible for processing the deacetylated 'detoxication label'. PLP requires a primary amino group, to which it binds to enable any possible PLP-dependent turnovers including b-elimination in lincomycin biosynthesis. Furthermore, based on the structures of lincomycin and celesticetin and 13 C incorporation experiments, we predict that homologous CcbF from celesticetin biosynthesis performs a different type of reaction, decarboxylation-dependent transamination, which results in preservation of the 2C residue allowing the attachment of the salicylate unit. Such a surprising nding that two homologous PLP-dependent proteins from highly related biosynthetic pathways should exhibit different reaction speci-city undoubtedly represents an exciting topic of further studies.