Formation of lipid and polymer based gold nanohybrids using a nanoreactor approach

Dominik Witzigmann; Sandro Sieber; Fabiola Porta; Philip Grossen; Andrej Bieri; Natalja Strelnikova; Thomas Pfohl; Cristina Prescianotto-Baschong; Jörg Huwyler

doi:10.1039/C5RA13967H

Formation of lipid and polymer based gold nanohybrids using a nanoreactor approach†

Dominik Witzigmann,^a Sandro Sieber,^a Fabiola Porta,^a Philip Grossen,^a Andrej Bieri,^b Natalja Strelnikova,^c Thomas Pfohl,^c Cristina Prescianotto-Baschong^d and Jörg Huwyler*^a

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* Corresponding authors

^a Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, Basel CH-4056, Switzerland
E-mail: joerg.huwyler@unibas.ch

^b Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Mattenstrasse 26, Basel CH-4058, Switzerland

^c Department of Chemistry, University of Basel, Klingelbergstrasse 80, Basel CH-4056, Switzerland

^d Biozentrum, University of Basel, Klingelbergstrasse 70, Basel CH-4056, Switzerland

Abstract

Nanocarriers encapsulating gold nanoparticles (AuNPs) hold tremendous promise for numerous biomedical applications. So far only a few fabrication strategies have been investigated and efficient processes for the manufacturing of gold nanohybrids (AuNHybs) are still missing. We encapsulated a tetrachloroaurate/citrate mixture within nanocarriers and initiated the AuNP formation after self-assembly of the nanomaterial by a temperature shift. This nanoreactor approach was successfully combined with the film-rehydration, nanoprecipitation, or microfluidics method. Different nanomaterials were validated including phospholipids and copolymers and the process was optimized towards encapsulation efficiency and physico-chemical homogeneity of AuNHybs. Our nanoreactor technology is versatile, efficient, and highly reproducible. Dynamic light scattering and electron microscopy techniques confirmed that generated lipid and polymer based AuNHybs were of uniform size below 130 nm and contained a single AuNP. The AuNHyb solutions had a deep-red color and exhibited the specific surface plasmon absorption of AuNPs. The unique optical properties of AuNHybs were used to visualize cellular uptake of nanocarriers in vitro demonstrating the promising applicability of AuNHybs as a bioimaging tool.

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DOI: https://doi.org/10.1039/C5RA13967H
Article type: Paper
Submitted: 15 Jul 2015
Accepted: 24 Aug 2015
First published: 25 Aug 2015

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RSC Adv., 2015,5, 74320-74328

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Formation of lipid and polymer based gold nanohybrids using a nanoreactor approach

D. Witzigmann, S. Sieber, F. Porta, P. Grossen, A. Bieri, N. Strelnikova, T. Pfohl, C. Prescianotto-Baschong and J. Huwyler, RSC Adv., 2015, 5, 74320 DOI: 10.1039/C5RA13967H

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Formation of lipid and polymer based gold nanohybrids using a nanoreactor approach†

Abstract

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Formation of lipid and polymer based gold nanohybrids using a nanoreactor approach

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