SCHEDULED MAINTENANCE
Linear scaffolds for multivalent targeting of melanocortin receptors†
Abstract
Molecules bearing one, two, three, or four copies of the tetrapeptide His-DPhe-Arg-Trp were attached to scaffolds based on ethylene glycol, glycerol, and D-mannitol by means of the copper-assisted azide–alkyne cyclization. The abilities of these compounds to block binding of a probe at the melanocortin 4 receptor were evaluated using a competitive binding assay. All of the multivalent molecules studied exhibited 30- to 40-fold higher apparent affinites when compared to a monovalent control. These results are consistent with divalent binding to receptor dimers. No evidence for tri- or tetravalent binding was obtained. Differences in the interligand spacing required for divalent binding, as opposed to tri- or tetravalent binding, may be responsible for these results.
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