Issue 19, 2015
From the journal:

Analytical Methods

Visual detection of 8-OHdG based on the aggregation of gold nanoparticles capped with the anti-8-OHdG antibody

Liping Wu,ab   Wendan Pu,b   Yue Liu,b   Huawen Zhao*b  and  Weiqun Shu*a  

* Corresponding authors

a Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, P. R. China
E-mail: xm0630@sina.com
Fax: +86-23-68772309
Tel: +86-23-68772309

b Department of Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, P. R. China
E-mail: sydzhw@aliyun.com
Fax: +86-23-68752217
Tel: +86-23-68752217

Abstract

Gold nanoparticles (AuNPs) were stably capped with the anti-8-OHdG polyclonal antibody. When 8-OHdG was added, the antibody-capped AuNPs would aggregate for the specific interaction of antibody and antigen. The maximum absorption wavelength of antibody-capped AuNPs red shifts gradually and the solution color changes from red to blue, with increasing concentration of 8-OHdG. Based on that, highly selective determination of 8-OHdG is set up, with a detection range of 0.25–5.00 μg mL−1 and LOD of 25 ng mL−1. The method is simple, rapid and easy to operate. Semi-quantitative determination can also be achieved by visual observation of the solution color change without any apparatus.

Graphical abstract: Visual detection of 8-OHdG based on the aggregation of gold nanoparticles capped with the anti-8-OHdG antibody

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Article information

DOI
https://doi.org/10.1039/C5AY01774B
Article type
Paper
Submitted
08 Jul 2015
Accepted
18 Aug 2015
First published
20 Aug 2015

Anal. Methods, 2015,7, 8360-8365

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Visual detection of 8-OHdG based on the aggregation of gold nanoparticles capped with the anti-8-OHdG antibody

L. Wu, W. Pu, Y. Liu, H. Zhao and W. Shu, Anal. Methods, 2015, 7, 8360 DOI: 10.1039/C5AY01774B

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