Issue 104, 2014
From the journal:

RSC Advances

Rapid and sensitive detection of acrylic acid using a novel fluorescence assay†

Zhi Yi Lee,ab   Sarada S. Raghavan,b   Farid J. Ghadessyb  and  Yin Nah Teo ORCID logo *ac  

* Corresponding authors

a Molecular Engineering Laboratory, Biomedical Sciences Institutes, A*STAR, 61 Biopolis Drive, #03-12 Proteos, Singapore 138673
E-mail: teoyn@bmsi.a-star.edu.sg

b p53 Laboratory, A*STAR, 8A Biomedical Grove, #06-06 Immunos, Singapore 138648

c Division of Chemistry and Biological Chemistry, SPMS, Nanyang Technological University, Singapore 637371

Abstract

We report a fluorescence-based method for the detection of acrylic acid, a high value chemical precursor of acrylate esters and polymers. Our method generates a fluorescent pyrazoline product in the presence of acrylic acid using photoactivated 1,3-dipolar cycloaddition with a diaryltetrazole-based probe, and is capable of detecting, as little as 36 ppb of acrylic acid in vitro and in vivo. We anticipate that this probe will be useful in high throughput screens for biosynthetic production of acrylic acid from biomass and can replace existing chromatographic methods of detection such as GCMS and LCMS, which are low throughput, time-consuming and require sample preparation.

Graphical abstract: Rapid and sensitive detection of acrylic acid using a novel fluorescence assay

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Article information

DOI
https://doi.org/10.1039/C4RA12495B
Article type
Communication
Submitted
16 Oct 2014
Accepted
29 Oct 2014
First published
05 Nov 2014

RSC Adv., 2014,4, 60216-60220

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Rapid and sensitive detection of acrylic acid using a novel fluorescence assay

Z. Y. Lee, S. S. Raghavan, F. J. Ghadessy and Y. N. Teo, RSC Adv., 2014, 4, 60216 DOI: 10.1039/C4RA12495B

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