Issue 18, 2014
From the journal:

Analytical Methods

Evaluating the antioxidant capacity of polyphenols with an off–on fluorescence probe and the mechanism study†

Linying Cao,ab   Hui Yu,ab   Shijun Shao,a   Shuai Wang*a  and  Yong Guo*a  

* Corresponding authors

a Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P. R. China
E-mail: guoyong@licp.cas.cn, shuaiw@licp.cas.cn
Fax: +86-931-8277088
Tel: +86 931 4968274

b University of Chinese Academy of Sciences, Beijing 100039, P. R. China

Abstract

A spin-labeled fluorescence probe (RNO˙) was employed to evaluate the antioxidant capacity of polyphenols. The hydrogen-transfer mechanism was proposed by kinetic studies using fluorescence spectroscopy, and the product was confirmed to be RNOH by HPLC-MS with little side products. The HPLC-UV/vis method devoted to the simultaneous monitoring of the RNO˙ and RNOH at 556 nm was developed, which can distinguish the antioxidant capacity of polyphenols with similar structure. The method has the potential to be used to screen antioxidants when modifying or optimizing natural antioxidants.

Graphical abstract: Evaluating the antioxidant capacity of polyphenols with an off–on fluorescence probe and the mechanism study

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Article information

DOI
https://doi.org/10.1039/C4AY01276C
Article type
Communication
Submitted
28 May 2014
Accepted
07 Jul 2014
First published
08 Jul 2014

Anal. Methods, 2014,6, 7149-7153

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Evaluating the antioxidant capacity of polyphenols with an off–on fluorescence probe and the mechanism study

L. Cao, H. Yu, S. Shao, S. Wang and Y. Guo, Anal. Methods, 2014, 6, 7149 DOI: 10.1039/C4AY01276C

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