A multiple amplification strategy for nucleic acid detection based on host–guest interaction between the β-cyclodextrin polymer and pyrene

Xiaochen Guo; Pei Liu; Xiaohai Yang; Kemin Wang; Qing Wang; Qiuping Guo; Jin Huang; Jianbo Liu; Chunxia Song; Wenshan Li

doi:10.1039/C4AN02402H

A multiple amplification strategy for nucleic acid detection based on host–guest interaction between the β-cyclodextrin polymer and pyrene†

Xiaochen Guo,^a Pei Liu,^b Xiaohai Yang,*^a Kemin Wang,*^a Qing Wang,^a Qiuping Guo,^a Jin Huang,^a Jianbo Liu,^a Chunxia Song^a and Wenshan Li^a

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* Corresponding authors

^a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
E-mail: kmwang@hnu.edu.cn, yangxiaohai@hnu.edu.cn
Fax: +86 731 88821566

^b Hunan Province Environmental Monitoring Centre, Changsha 410014, China

Abstract

A multiple amplification strategy has been developed for nucleic acid detection based on host–guest interaction between the β-cyclodextrin polymer (β-CDP) and pyrene. Briefly, the detection system consists of three parts: the polymerase and nicking enzyme-assisted isothermal strand displacement amplification (SDA) activated by a target DNA or microRNA; the exonuclease III-aided cyclic enzymatic amplification (CEA); and the fluorescence enhancement effect based on host–guest interaction between β-CDP and pyrene. This strategy showed a good positive linear correlation with target DNA concentrations in the range from 75 fM to 1 pM with a detection limit of 41 fM. Significantly, our amplification platform was further validated and evaluated successfully by assaying miRNA-21 in human serum. The proposed assay has great potential as a nucleic acid quantification method for use in biomedical research, clinical analysis and disease diagnostics.

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A multiple amplification strategy for nucleic acid detection based on host–guest interaction between the β-cyclodextrin polymer and pyrene†

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A multiple amplification strategy for nucleic acid detection based on host–guest interaction between the β-cyclodextrin polymer and pyrene

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