Issue 62, 2013
From the journal:

Chemical Communications

Solid phase click ligation for the synthesis of very long oligonucleotides†

Jieqiong Qiu,a   Afaf H. El-Sagheerab  and  Tom Brown*a  

* Corresponding authors

a School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK
E-mail: tb2@soton.ac.uk
Fax: +44 (0)2380 592991
Tel: +44 (0)2380 592974

b Chemistry Branch, Dept. of Science and Mathematics, Suez University, Suez 43721, Egypt

Abstract

Oligonucleotides have been ligated efficiently on solid-phase using CuAAC and SPAAC chemistry to produce up to 186-mer triazole linked DNA products. Multiple sequential ligation reactions can be carried out by using a masked azide approach. This work suggests a novel modular approach to the synthesis of large complex oligonucleotide analogues.

Graphical abstract: Solid phase click ligation for the synthesis of very long oligonucleotides

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Article information

DOI
https://doi.org/10.1039/C3CC42451K
Article type
Communication
Submitted
04 Apr 2013
Accepted
21 Jun 2013
First published
21 Jun 2013
This article is Open Access
Creative Commons BY license

Chem. Commun., 2013,49, 6959-6961

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Solid phase click ligation for the synthesis of very long oligonucleotides

J. Qiu, A. H. El-Sagheer and T. Brown, Chem. Commun., 2013, 49, 6959 DOI: 10.1039/C3CC42451K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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