Issue 19, 2013
From the journal:

Analytical Methods

Label-free electrochemical impedance biosensor for sequence-specific recognition of double-stranded DNA

Hanye Zheng,a   Xiaoming Ma,a   Lisong Chen,a   Zhenyu Lin,*a   Longhua Guo,a   Bin Qiua  and  Guonan Chena  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, China
E-mail: zylin@fzu.edu.cn
Fax: +86-591-22866135

Abstract

A simple and label-free DNA electrochemical impedance biosensor has been developed for the sequence-specific recognition of double-stranded DNA (ds-DNA). The probe triplex-forming oligonucleotide (TFO) is immobilized on the gold electrode through Au–S interaction. In the presence of the target ds-DNA, the probe TFO interacted with the bases in the major groove of the ds-DNA to form a rigid triplex structure via Hoogsteen hydrogen bonds, leading to an increase of the electron-transfer resistance. It is found that the change of impedance has a linear relationship with the concentration of the target in the range of 0.1–40 nmol L−1, and the detection limit is as low as 0.04 nmol L−1 (S/N = 3).

Graphical abstract: Label-free electrochemical impedance biosensor for sequence-specific recognition of double-stranded DNA

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Article information

DOI
https://doi.org/10.1039/C3AY40972D
Article type
Paper
Submitted
14 Jun 2013
Accepted
16 Jul 2013
First published
16 Jul 2013

Anal. Methods, 2013,5, 5005-5009

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Label-free electrochemical impedance biosensor for sequence-specific recognition of double-stranded DNA

H. Zheng, X. Ma, L. Chen, Z. Lin, L. Guo, B. Qiu and G. Chen, Anal. Methods, 2013, 5, 5005 DOI: 10.1039/C3AY40972D

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