Issue 20, 2013
From the journal:

Analytical Methods

Homogeneous immunoassay of cortisol based on microchip electrophoresis with chemiluminescence detection

Fanggui Ye,a   Tinzhen Yang,a   Yong Huang,a   Shutin Lia  and  Shulin Zhao*a  

* Corresponding authors

a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China
E-mail: zhaoshulin001@163.com
Fax: +86 773 5832294
Tel: +86 773 5856104

Abstract

An homogeneous immunoassay based on microchip electrophoresis (MCE) separation and chemiluminescence (CL) detection was developed for the determination of cortisol. The proposed method deployed the competitive immunoreaction of cortisol and horseradish peroxidase-labeled cortisol (HRP-cortisol) with a limited amount of anti-cortisol mouse monoclonal antibody (Ab). Free and bound HRP-cortisol were then separated by MCE. The effect of various factors such as conditions for the CL reaction, MCE separation and incubation time for the immunoreactions were examined and optimized. Under the optimal assay conditions, the MCE separation was accomplished within 60 s. Highly sensitive CL detection was achieved by means of HPR-catalyzed luminol–H2O2 reaction. The linear range for cortisol detection was 9–900 nM, and the limit of detection was 4.2 nM (S/N = 3). The suitability of the proposed method for the quantification of cortisol in human serum was demonstrated.

Graphical abstract: Homogeneous immunoassay of cortisol based on microchip electrophoresis with chemiluminescence detection

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Article information

DOI
https://doi.org/10.1039/C3AY40949J
Article type
Paper
Submitted
10 Jun 2013
Accepted
09 Aug 2013
First published
09 Aug 2013

Anal. Methods, 2013,5, 5657-5661

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Homogeneous immunoassay of cortisol based on microchip electrophoresis with chemiluminescence detection

F. Ye, T. Yang, Y. Huang, S. Li and S. Zhao, Anal. Methods, 2013, 5, 5657 DOI: 10.1039/C3AY40949J

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