Issue 7, 2014
From the journal:

Analyst

Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin†

Jayeon Lee,ab   Chang Hoon Jeon,ab   Sang Jung Ahnc  and  Tai Hwan Ha*ab  

* Corresponding authors

a Nanobiotechnology (Major), School of Engineering, University of Science & Technology Centre, Daejeon 305-806, Republic of Korea
E-mail: taihwan@kribb.re.kr
Fax: +82 42 879 8596
Tel: +82 42 860 4272

b Research center of integrative cellolomics, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea
E-mail: taihwan@kribb.re.kr
Fax: +82 42 879 8596
Tel: +82 42 860 4272

c Center for Nano-Imaging Technology, Division of Industrial Metrology, Korea Research Institute of Standards and Science (KRISS), Yuseong-gu, Daejeon 305-340, Republic of Korea

Abstract

Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5′-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an ∼1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.

Graphical abstract: Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

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Article information

DOI
https://doi.org/10.1039/C3AN01639K
Article type
Communication
Submitted
30 Aug 2013
Accepted
13 Jan 2014
First published
14 Jan 2014

Analyst, 2014,139, 1622-1627

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Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

J. Lee, C. H. Jeon, S. J. Ahn and T. H. Ha, Analyst, 2014, 139, 1622 DOI: 10.1039/C3AN01639K

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