Studies of polyketide chain-assembly processes: origins of the hydrogen and oxygen atoms in colletodiol
Abstract
The Origins of all the oxygen and hydrogen atoms in colletodiol (2) have been elucidated by incorporation of label from [1-13C,18O2]- and [1-13C,2H3]-acetate and 18O2 gas into (2) in clusters of Cytospora sp.(ATCC 20502); from the resultant labelling pattern the structures of the enzyme-bound precursors can be deduced and information obtained on the processes occurring during the early stages of polyketide chain-assembly.